PMID- 12464391
OWN - NLM
STAT- MEDLINE
DCOM- 20030828
LR  - 20190922
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 15
IP  - 2
DP  - 2003 Feb
TI  - Endothelin-1 stimulated capacitative Ca2+ entry through ET(A) receptors of a rat 
      brain-derived type-1 astrocyte cell line, IA-1g1.
PG  - 197-207
AB  - The present study demonstrated that endotheline-1 (ET-1) stimulated a biphasic 
      (transient and sustained) increase in [Ca(2+)](i) and signaling was blocked by 
      BQ123 and inhibited by BQ788. RT-PCR analysis revealed that ET(A) was expressed 
      more than ET(B) mRNA-suggesting that ET(A) is the major receptor. Simply 
      reintroducing Ca(2+) in the buffer stimulated a sustained increase in [Ca(2+)](i) 
      and the effect was inhibited by U73122, thapsigargin (TG), miconazole and 
      SKF96365. When measured in Ca(2+)-free buffer, the ET-1-stimulated Ca(2+) 
      transient decreased by 73% and the reintroduction of Ca(2+) induced a large 
      sustained increase in [Ca(2+)](i). These effects were not affected by nifedipine, 
      but were inhibited by miconazole and SKF96365-indicating that the sustained 
      increase in [Ca(2+)](i) mediated by ET-1 was mostly due to capacitative Ca(2+) 
      entry (CCE). The ET-1-induced CCE was inhibited by phorbol ester (PMA) but was 
      enhanced by GF109203X; it was also enhanced by 8-bromo-cyclic AMP (8-Br-cAMP) but 
      was inhibited by H89. Thus, protein kinase C (PKC) negatively regulated and 
      cAMP-dependent protein kinase (PKA) positively regulated the ET-1-mediated CCE in 
      these cells.
FAU - Ju, You Jing
AU  - Ju YJ
AD  - Institute of Neuroscience, College of Life Science, National Yang Ming 
      University, #155, Section 2, Li-Non Street, Shi-Pai, Taipei, Taiwan, ROC.
FAU - Wang, Chia-Mei
AU  - Wang CM
FAU - Hung, Amos C
AU  - Hung AC
FAU - Lo, Jun-Chih
AU  - Lo JC
FAU - Lin, Hung-Jung
AU  - Lin HJ
FAU - Sun, Synthia H
AU  - Sun SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Amino Acids, Cyclic)
RN  - 0 (Antihypertensive Agents)
RN  - 0 (Endothelin-1)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (Receptor, Endothelin A)
RN  - 0 (Receptor, Endothelin B)
RN  - 0 (Receptors, Endothelin)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - S2A8YZM151 (cyclo(Trp-Asp-Pro-Val-Leu))
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acids, Cyclic/metabolism
MH  - Animals
MH  - Antihypertensive Agents/pharmacology
MH  - Astrocytes/cytology/*metabolism
MH  - Brain/cytology
MH  - Calcium/*pharmacokinetics
MH  - Calcium Signaling/*drug effects/*physiology
MH  - Cell Line
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Electric Capacitance
MH  - Endothelin-1/*pharmacology
MH  - Homeostasis/drug effects/physiology
MH  - Peptides, Cyclic/pharmacology
MH  - Protein Kinase C/metabolism
MH  - Rats
MH  - Receptor, Endothelin A
MH  - Receptor, Endothelin B
MH  - Receptors, Endothelin/*metabolism
EDAT- 2002/12/05 04:00
MHDA- 2003/08/29 05:00
CRDT- 2002/12/05 04:00
PHST- 2002/12/05 04:00 [pubmed]
PHST- 2003/08/29 05:00 [medline]
PHST- 2002/12/05 04:00 [entrez]
AID - S0898656802000797 [pii]
AID - 10.1016/s0898-6568(02)00079-7 [doi]
PST - ppublish
SO  - Cell Signal. 2003 Feb;15(2):197-207. doi: 10.1016/s0898-6568(02)00079-7.