PMID- 12466101
OWN - NLM
STAT- MEDLINE
DCOM- 20030221
LR  - 20190513
IS  - 0305-7364 (Print)
IS  - 1095-8290 (Electronic)
IS  - 0305-7364 (Linking)
VI  - 90
IP  - 5
DP  - 2002 Nov
TI  - Fructokinase and hexokinase from pollen grains of bell pepper (Capsicum annuum 
      L.): possible role in pollen germination under conditions of high temperature and 
      CO2 enrichment.
PG  - 607-12
AB  - The processes of pollen grain development and germination depend on the uptake 
      and metabolism of pollen sugars. In pepper (Capsicum annuum L.), initial sugar 
      metabolism includes sucrose hydrolysis by invertase and subsequent 
      phosphorylation of glucose and fructose by hexose kinases. The main objective of 
      this study was to investigate changes in fructokinase (EC 2.7.1.4) and hexokinase 
      (EC.2.7.1.1) activities in pepper flowers during their development, and to study 
      the possible roles of these enzymes in determining pollen germination capacity 
      under high temperature and under CO(2) enrichment, previously shown to modify 
      sugar concentrations in pepper pollen (Aloni et al., 2001 Physiologia Plantarum 
      112: 505-512). Fructokinase (FK) activity was predominant in pepper pollen, and 
      increased during pollen maturation. Pollen hexokinase (HK) activity was low and 
      did not change throughout pollen development. High-temperature treatment 
      (day/night, 32/26 degrees C) of pepper plants reduced the percentage of pollen 
      that germinated compared with that under normal temperatures (26/22 degrees C), 
      and concomitantly reduced the activity of FK in mature pollen. High temperature 
      also reduced FK and HK activity in the anther. Under high ambient CO(2) (800 
      micro l l(-1)) pollen FK activity was enhanced. The results suggest that pollen 
      and anther FK may play a role in the regulation of pollen germination, possibly 
      by providing fructose-6-phosphate for glycolysis, or through conversion to 
      UDP-glucose (UDPG) to support the biosynthesis of cell wall material for pollen 
      tube growth. High temperature stress and CO(2) enrichment may influence pollen 
      germination capacity by affecting these pathways.
FAU - Karni, Leah
AU  - Karni L
AD  - Department of Vegetable Crops, Institute of Field and Garden Crops, A.R.O., The 
      Volcani Center, Bet Dagan, 50250 Israel.
FAU - Aloni, Beny
AU  - Aloni B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Ann Bot
JT  - Annals of botany
JID - 0372347
RN  - 02F3473H9O (Magnesium Chloride)
RN  - 142M471B3J (Carbon Dioxide)
RN  - EC 2.7.1.- (Fructokinases)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.4 (fructokinase)
RN  - M4I0D6VV5M (Calcium Chloride)
SB  - IM
MH  - Calcium Chloride/pharmacology
MH  - Capsicum/drug effects/*enzymology/growth & development/metabolism
MH  - Carbohydrate Metabolism
MH  - Carbon Dioxide/*metabolism/pharmacology
MH  - Flowers/drug effects/enzymology
MH  - Fructokinases/*metabolism
MH  - *Germination/drug effects
MH  - Hexokinase/*metabolism
MH  - *Hot Temperature
MH  - Magnesium Chloride/pharmacology
MH  - Pollen/drug effects/*enzymology/growth & development/metabolism
MH  - Time Factors
PMC - PMC4240450
EDAT- 2002/12/06 04:00
MHDA- 2003/02/22 04:00
PMCR- 2003/11/01
CRDT- 2002/12/06 04:00
PHST- 2002/12/06 04:00 [pubmed]
PHST- 2003/02/22 04:00 [medline]
PHST- 2002/12/06 04:00 [entrez]
PHST- 2003/11/01 00:00 [pmc-release]
AID - 10.1093/aob/mcf234 [doi]
PST - ppublish
SO  - Ann Bot. 2002 Nov;90(5):607-12. doi: 10.1093/aob/mcf234.