PMID- 12467029
OWN - NLM
STAT- MEDLINE
DCOM- 20030519
LR  - 20061115
IS  - 1059-910X (Print)
IS  - 1059-910X (Linking)
VI  - 59
IP  - 6
DP  - 2002 Dec 15
TI  - System for combined three-dimensional morphological and molecular analysis of 
      thick tissue specimens.
PG  - 522-30
AB  - We present a new system for simultaneous morphological and molecular analysis of 
      thick tissue samples. The system is composed of a computer-assisted microscope 
      and a JAVA-based image display, analysis, and visualization program that allows 
      acquisition, annotation, meaningful storage, three-dimensional reconstruction, 
      and analysis of structures of interest in thick sectioned tissue specimens. We 
      describe the system in detail and illustrate its use by imaging, reconstructing, 
      and analyzing two complete tissue blocks that were differently processed and 
      stained. One block was obtained from a ductal carcinoma in situ (DCIS) lumpectomy 
      specimen and stained alternatively with Hematoxilyn and Eosin (H&E), and with a 
      counterstain and fluorescence in situ hybridization (FISH) to the ERB-B2 gene. 
      The second block contained a fully sectioned mammary gland of a mouse, stained 
      for histology with H&E. We show how the system greatly reduces the amount of 
      interaction required for the acquisition and analysis and is, therefore, suitable 
      for studies that require morphologically driven, wide-scale (e.g., whole gland) 
      analysis of complex tissue samples or cultures.
FAU - Fernandez-Gonzalez, Rodrigo
AU  - Fernandez-Gonzalez R
AD  - Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, 
      California 94720, USA.
FAU - Jones, Arthur
AU  - Jones A
FAU - Garcia-Rodriguez, Enrique
AU  - Garcia-Rodriguez E
FAU - Chen, Ping Yuan
AU  - Chen PY
FAU - Idica, Adam
AU  - Idica A
FAU - Lockett, Stephen J
AU  - Lockett SJ
FAU - Barcellos-Hoff, Mary Helen
AU  - Barcellos-Hoff MH
FAU - Ortiz-De-Solorzano, Carlos
AU  - Ortiz-De-Solorzano C
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Review
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (Oncogene Proteins)
SB  - IM
MH  - Animals
MH  - Breast Neoplasms/*chemistry/pathology
MH  - Female
MH  - Humans
MH  - Image Cytometry/instrumentation/*methods
MH  - Image Processing, Computer-Assisted/*methods
MH  - Mice
MH  - Microcomputers
MH  - Microscopy, Confocal/instrumentation/methods
MH  - Oncogene Proteins/analysis/genetics
MH  - Software
MH  - User-Computer Interface
RF  - 9
OTO - NASA
OT  - Non-programmatic
EDAT- 2002/12/06 04:00
MHDA- 2003/05/20 05:00
CRDT- 2002/12/06 04:00
PHST- 2002/12/06 04:00 [pubmed]
PHST- 2003/05/20 05:00 [medline]
PHST- 2002/12/06 04:00 [entrez]
AID - 10.1002/jemt.10233 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2002 Dec 15;59(6):522-30. doi: 10.1002/jemt.10233.