PMID- 12467363
OWN - NLM
STAT- MEDLINE
DCOM- 20030509
LR  - 20190916
IS  - 1031-3613 (Print)
IS  - 1031-3613 (Linking)
VI  - 14
IP  - 5-6
DP  - 2002
TI  - Simultaneous detection of viability and sex of bovine spermatozoa.
PG  - 373-6
AB  - The viability and sex of bovine spermatozoa were simultaneously evaluated. After 
      viability and acrosome staining with trypan blue/Giemsa, only live spermatozoa 
      became decondensed by a modified papain-dithiothreitol method. Owing to this 
      specific effect, live sperm heads were easily distinguished by their enlarged 
      size and dark violet colour from small, light blue dead sperm heads. In the same 
      sperm sample, X- and Y-chromosome-bearing sperm were distinguished by their 
      fluorescent signal, using fluorescence in situ hybridization (FISH) with an XY 
      paint set and 4,6-diamino-2-phenylindole counterstaining. The combined staining 
      provides a method for morphological and viability evaluation before FISH and 
      permits identification of the proportions of X- and Y-chromosome-containing live 
      spermatozoa in a semen sample. However, only 25% of the undecondensed dead sperm 
      express signals allowing detection of the sex of the chromosome. The method may 
      be an effective tool in evaluating sex-oriented semen samples.
FAU - Revay, T
AU  - Revay T
AD  - Budapest University of Technology and Economics, Department of Biochemistry and 
      Food Technology, Hungary.
FAU - Kovacs, A
AU  - Kovacs A
FAU - Rens, W
AU  - Rens W
FAU - Gustavsson, I
AU  - Gustavsson I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Animals
MH  - *Cattle
MH  - *Cell Survival
MH  - Fluorescent Dyes
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles
MH  - Male
MH  - Sex Determination Analysis/*veterinary
MH  - Spermatozoa/*physiology/ultrastructure
MH  - Staining and Labeling
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 2002/12/07 04:00
MHDA- 2003/05/13 05:00
CRDT- 2002/12/07 04:00
PHST- 2002/04/23 00:00 [received]
PHST- 2002/08/13 00:00 [accepted]
PHST- 2002/12/07 04:00 [pubmed]
PHST- 2003/05/13 05:00 [medline]
PHST- 2002/12/07 04:00 [entrez]
AID - RD02026 [pii]
AID - 10.1071/rd02026 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2002;14(5-6):373-6. doi: 10.1071/rd02026.