PMID- 12467539
OWN - NLM
STAT- MEDLINE
DCOM- 20030117
LR  - 20131121
IS  - 1009-2587 (Print)
IS  - 1009-2587 (Linking)
VI  - 18
IP  - 4
DP  - 2002 Aug
TI  - [An experimental study of the changes of rat hepatocytic glycolysis during 
      hypoxia].
PG  - 238-41
AB  - OBJECTIVE: To investigate the effects of hypoxia on the glycolysis in cultured 
      rat hepatocytes. METHODS: Mixed gas with different concentrations of O(2), CO(2) 
      and N(2) was prepared for the in vitro culture of normal rat hepatocytes. The 
      cell strains were set to be A, B, C groups, which were observed at 1, 2, 4, 8 and 
      16 hours after hypoxia with normal hepatocytes as the control. Biochemical 
      methods were employed to determine the activities of the key enzymes during 
      hepatocytic glycolysis such as hexokinase (HK), phosphofructokinase (PFK), 
      pyruvate kinase (PK), lactate dehydrogenase (LDH) and the change of the content 
      of lactic acid (LA) in the culture fluid. RESULTS: (1) The LDH activity of the 
      rat hepatocytes increased significantly at all the time points of hypoxia in A 
      and B groups when compared with that in control group (P < 0.05), while the 
      activity increased obviously in C group since 2 hours after hypoxia (P < 0.05). 
      (2) The HK activity of the cells in A group increased significantly at 1, 2, 4 
      and 16 hours after hypoxia and that in B and C groups increased obviously at 1 
      hour when compared with control group (P < 0.05). While the cellular PFK activity 
      in A group increased markedly at 1 and 4 hours after hypoxia and that in B and C 
      groups increased evidently at 4 hours after hypoxia (P < 0.05). The cellular PK 
      activity in all the three groups increased at all the hypoxic time points (P < 
      0.05). (3) The cellular LA content in A and B groups began to increase since 2 
      hours and that in C group did so since 4 hours after hypoxia and increased along 
      with the time lapse (P < 0.05). CONCLUSION: hypoxia might initiate glycolysis.
FAU - Ma, Zhengwei
AU  - Ma Z
AD  - Institute of Burn Research, Southwestern Hospital, Third Military Medical 
      University, Chongqing 400038, P.R. China.
FAU - Wang, Shiliang
AU  - Wang S
FAU - Wang, Fengjun
AU  - Wang F
FAU - Wang, Pei
AU  - Wang P
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Shao Shang Za Zhi
JT  - Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns
JID - 100959418
RN  - 33X04XA5AT (Lactic Acid)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.7.1 - (Phosphofructokinases)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - *Glycolysis
MH  - Hepatocytes/enzymology/*metabolism
MH  - Hexokinase/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Lactic Acid/metabolism
MH  - Oxygen/*metabolism
MH  - Phosphofructokinases/metabolism
MH  - Pyruvate Kinase/metabolism
MH  - Rats
EDAT- 2002/12/07 04:00
MHDA- 2003/01/18 04:00
CRDT- 2002/12/07 04:00
PHST- 2002/12/07 04:00 [pubmed]
PHST- 2003/01/18 04:00 [medline]
PHST- 2002/12/07 04:00 [entrez]
PST - ppublish
SO  - Zhonghua Shao Shang Za Zhi. 2002 Aug;18(4):238-41.