PMID- 12468722
OWN - NLM
STAT- MEDLINE
DCOM- 20030318
LR  - 20240104
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 14
IP  - 12
DP  - 2002 Dec
TI  - A high-throughput Arabidopsis reverse genetics system.
PG  - 2985-94
AB  - A collection of Arabidopsis lines with T-DNA insertions in known sites was 
      generated to increase the efficiency of functional genomics. A high-throughput 
      modified thermal asymmetric interlaced (TAIL)-PCR protocol was developed and used 
      to amplify DNA fragments flanking the T-DNA left borders from approximately 
      100000 transformed lines. A total of 85108 TAIL-PCR products from 52964 T-DNA 
      lines were sequenced and compared with the Arabidopsis genome to determine the 
      positions of T-DNAs in each line. Predicted T-DNA insertion sites, when mapped, 
      showed a bias against predicted coding sequences. Predicted insertion mutations 
      in genes of interest can be identified using Arabidopsis Gene Index name searches 
      or by BLAST (Basic Local Alignment Search Tool) search. Insertions can be 
      confirmed by simple PCR assays on individual lines. Predicted insertions were 
      confirmed in 257 of 340 lines tested (76%). This resource has been named SAIL 
      (Syngenta Arabidopsis Insertion Library) and is available to the scientific 
      community at www.tmri.org.
FAU - Sessions, Allen
AU  - Sessions A
AD  - Torrey Mesa Research Institute, Syngenta, 3115 Merryfield Row, San Diego, 
      California 92121, USA. allen.sessions@syngenta.com
FAU - Burke, Ellen
AU  - Burke E
FAU - Presting, Gernot
AU  - Presting G
FAU - Aux, George
AU  - Aux G
FAU - McElver, John
AU  - McElver J
FAU - Patton, David
AU  - Patton D
FAU - Dietrich, Bob
AU  - Dietrich B
FAU - Ho, Patrick
AU  - Ho P
FAU - Bacwaden, Johana
AU  - Bacwaden J
FAU - Ko, Cynthia
AU  - Ko C
FAU - Clarke, Joseph D
AU  - Clarke JD
FAU - Cotton, David
AU  - Cotton D
FAU - Bullis, David
AU  - Bullis D
FAU - Snell, Jennifer
AU  - Snell J
FAU - Miguel, Trini
AU  - Miguel T
FAU - Hutchison, Don
AU  - Hutchison D
FAU - Kimmerly, Bill
AU  - Kimmerly B
FAU - Mitzel, Theresa
AU  - Mitzel T
FAU - Katagiri, Fumiaki
AU  - Katagiri F
FAU - Glazebrook, Jane
AU  - Glazebrook J
FAU - Law, Marc
AU  - Law M
FAU - Goff, Stephen A
AU  - Goff SA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Plant)
RN  - 0 (T-DNA)
SB  - IM
MH  - Agrobacterium tumefaciens/genetics
MH  - Arabidopsis/*genetics
MH  - Binding Sites/genetics
MH  - Chromosomes, Plant/genetics
MH  - DNA, Bacterial/chemistry/*genetics
MH  - DNA, Plant/chemistry/genetics
MH  - Databases, Genetic
MH  - Genome, Plant
MH  - Internet
MH  - Mutagenesis, Insertional
MH  - Plants, Genetically Modified
MH  - Polymerase Chain Reaction/methods
MH  - Seeds/genetics
MH  - Sequence Analysis, DNA
PMC - PMC151197
EDAT- 2002/12/07 04:00
MHDA- 2003/03/19 04:00
PMCR- 2003/12/01
CRDT- 2002/12/07 04:00
PHST- 2002/12/07 04:00 [pubmed]
PHST- 2003/03/19 04:00 [medline]
PHST- 2002/12/07 04:00 [entrez]
PHST- 2003/12/01 00:00 [pmc-release]
AID - 004630 [pii]
AID - 10.1105/tpc.004630 [doi]
PST - ppublish
SO  - Plant Cell. 2002 Dec;14(12):2985-94. doi: 10.1105/tpc.004630.