PMID- 12469196
OWN - NLM
STAT- MEDLINE
DCOM- 20030616
LR  - 20181130
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 22
IP  - 1
DP  - 2003 Jan
TI  - Protein kinase C-alpha activation by phorbol ester induces secretion of 
      gelatinase B/MMP-9 through ERK 1/2 pathway in capillary endothelial cells.
PG  - 137-43
AB  - In the present study, phorbol 12-myristate 13-acetate (PMA) was found to increase 
      secretion of matrix metalloproteinase (MMP)-9 and in vitro invasion in bovine 
      capillary endothelial (BCE) cells, which were blocked by specific inhibitors of 
      protein kinase C (PKC). To elucidate molecular mechanisms involved, we studied 
      the effect of PMA on the activation of mitogen activated protein kinases (MAPKs), 
      and found that PMA activated extracellular signal-regulated kinase (ERK)1/2 and 
      PD98059, a specific inhibitor of MAPK kinase, significantly reduced PMA-induced 
      MMP-9 secretion as well as in vitro invasion of BCE cells. Treatment of safingol, 
      a specific PKC-alpha inhibitor, and introduction of antisense PKC-alpha into 
      these cells reduced the secretion of MMP-9 and activation of ERK1/2 by PMA. 
      Furthermore, we employed adenoviral PKC-alpha and found that weak PMA stimulation 
      (5 ng/ml) enhanced ERK1/2 activation and MMP-9 secretion in these cells. 
      Therefore, we strongly suggest that PKC-alpha, partly at least, have a crucial 
      role in MMP-9 secretion and invasion of BCE cells which are mediated via ERK1/2 
      signaling pathway.
FAU - Park, Myung-Jin
AU  - Park MJ
AD  - Laboratory of Cell Biology, Korea Cancer Center Hospital, 139-706 Seoul, Korea.
FAU - Park, In-Chul
AU  - Park IC
FAU - Lee, Hyung-Chan
AU  - Lee HC
FAU - Woo, Sang-Hyeok
AU  - Woo SH
FAU - Lee, Jae-Young
AU  - Lee JY
FAU - Hong, Young-Joon
AU  - Hong YJ
FAU - Rhee, Chang-Hun
AU  - Rhee CH
FAU - Lee, Yun-Sil
AU  - Lee YS
FAU - Lee, Seung-Hoon
AU  - Lee SH
FAU - Shim, Bum-Sang
AU  - Shim BS
FAU - Kuroki, Toshino
AU  - Kuroki T
FAU - Hong, Seok-Il
AU  - Hong SI
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Flavonoids)
RN  - 0 (Imidazoles)
RN  - 0 (Pyridines)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (Protein Kinase C-alpha)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - OU13V1EYWQ (SB 203580)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Animals
MH  - Capillaries/cytology/enzymology
MH  - Cattle
MH  - Endothelium, Vascular/cytology/*enzymology
MH  - Enzyme Activation
MH  - Flavonoids/pharmacology
MH  - Imidazoles/pharmacology
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Mitogen-Activated Protein Kinase 1/*physiology
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinases/*physiology
MH  - Protein Kinase C/*physiology
MH  - Protein Kinase C-alpha
MH  - Pyridines/pharmacology
MH  - Tetradecanoylphorbol Acetate/*pharmacology
EDAT- 2002/12/07 04:00
MHDA- 2003/06/17 05:00
CRDT- 2002/12/07 04:00
PHST- 2002/12/07 04:00 [pubmed]
PHST- 2003/06/17 05:00 [medline]
PHST- 2002/12/07 04:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2003 Jan;22(1):137-43.