PMID- 12469315
OWN - NLM
STAT- MEDLINE
DCOM- 20030423
LR  - 20191106
IS  - 0022-1260 (Print)
IS  - 0022-1260 (Linking)
VI  - 48
IP  - 1
DP  - 2002 Feb
TI  - Polyphasic approaches to the identification of predominant 
      polyphosphate-accumulating organisms in a laboratory-scale anaerobic/aerobic 
      activated sludge system.
PG  - 43-54
AB  - By combination of denaturing gradient gel electrophoresis of PCR-amplified 16S 
      rDNA (PCR-DGGE), quinone profiling, and 16S rRNA-targeted fluorescence in situ 
      hybridization (FISH), a polyphosphate-accumulating organism (PAO) responsible for 
      phosphate (P)-removal was identified in activated sludge with high P-removal 
      ability from a laboratory-scale anaerobic/aerobic continuous flow reactor. The 
      DNA fragment from the most dense band on the DGGE gel was closely related to that 
      of 'Candidatus Accumulibacter phosphatis' (beta-Proteobacteria). Quinone 
      profiling also suggested the predominance of beta-Proteobacteria. FISH with a 
      specific oligonucleotide probe designed for the sequence showed that the targeted 
      bacterium was dominant in the activated sludge, and the accumulation and 
      consumption of polyphosphate were observed by dual staining with 
      4',6-diamidino-2-phenylindole. The bacterium was concluded to be the responsible 
      PAO in the reactor. However, when the P-removal ability per cell slightly 
      decreased, the dominance of the PAO greatly diminished in the activated sludge. 
      Such sludge might be dominated by other types of PAOs.
FAU - Onda, Shin
AU  - Onda S
AD  - Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 
      192-0397, Japan.
FAU - Hiraishi, Akira
AU  - Hiraishi A
FAU - Matsuo, Yoshitaka
AU  - Matsuo Y
FAU - Takii, Susumu
AU  - Takii S
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Gen Appl Microbiol
JT  - The Journal of general and applied microbiology
JID - 0165543
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 0 (Polyphosphates)
RN  - 0 (Quinones)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Base Sequence
MH  - Betaproteobacteria/genetics/isolation & purification/*metabolism
MH  - Bioreactors
MH  - DNA, Bacterial/chemistry/genetics
MH  - Electrophoresis
MH  - Fluorescent Dyes/chemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles/chemistry
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Polyphosphates/*metabolism
MH  - Quinones/analysis
MH  - RNA, Ribosomal, 16S/chemistry/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid/*methods
EDAT- 2002/12/07 04:00
MHDA- 2003/04/24 05:00
CRDT- 2002/12/07 04:00
PHST- 2002/12/07 04:00 [pubmed]
PHST- 2003/04/24 05:00 [medline]
PHST- 2002/12/07 04:00 [entrez]
AID - 10.2323/jgam.48.43 [doi]
PST - ppublish
SO  - J Gen Appl Microbiol. 2002 Feb;48(1):43-54. doi: 10.2323/jgam.48.43.