PMID- 12469948
OWN - NLM
STAT- MEDLINE
DCOM- 20030310
LR  - 20190922
IS  - 1061-4303 (Print)
IS  - 1061-4303 (Linking)
VI  - 74
IP  - 5
DP  - 2002 Sep-Oct
TI  - Microbial community structures in foaming and nonfoaming full-scale wastewater 
      treatment plants.
PG  - 437-49
AB  - A survey of full-scale activated-sludge plants in Illinois revealed that 
      filamentous foaming is a widespread problem in the state, and that the causes and 
      consequences of foaming control strategies are not fully understood. To link 
      microbial community structure to foam occurrence, microbial populations in eight 
      foaming and nine nonfoaming full-scale activated-sludge systems were quantified 
      using oligonucleotide hybridization probes targeting the ribosomal RNA (rRNA) of 
      the mycolata; Gordonia spp.; Gordonia amarae; "Candidatus Microthrix parvicella"; 
      the alpha-, beta-, and gamma-subclasses of the Proteobacteria, and members of the 
      Cytophaga-Flavobacteria. Parallel measurements of microbial population abundance 
      using hybridization of extracted RNA and fluorescence in situ hybridization 
      (FISH) showed that the levels of mycolata, particularly Gordonia spp., were 
      higher in most foaming systems compared with nonfoaming systems. Fluorescence in 
      situ hybridization and microscopy suggested the involvement of "Candidatus 
      Microthrix parvicella" and Skermania piniformis in foam formation in other 
      plants. Finally, high numbers of "Candidatus Microthrix parvicella" were detected 
      by FISH in foam and mixed liquor samples of one plant, whereas the corresponding 
      levels of rRNA were low. This finding implies that inactive "Candidatus 
      Microthrix parvicella" cells (i.e., cells with low rRNA levels) can cause 
      foaming.
FAU - de los Reyes, Francis L 3rd
AU  - de los Reyes FL 3rd
AD  - University of Illinois at Urbana-Champaign, USA.
FAU - Rothauszky, Dagmar
AU  - Rothauszky D
FAU - Raskin, Lutgarde
AU  - Raskin L
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Water Environ Res
JT  - Water environment research : a research publication of the Water Environment 
      Federation
JID - 9886167
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (Surface-Active Agents)
SB  - IM
MH  - Bacteria
MH  - DNA Primers
MH  - DNA, Bacterial/*genetics
MH  - Data Collection
MH  - In Situ Hybridization, Fluorescence
MH  - Population Dynamics
MH  - RNA, Ribosomal
MH  - Surface-Active Agents
MH  - *Waste Disposal, Fluid
MH  - *Water Microbiology
EDAT- 2002/12/10 04:00
MHDA- 2003/03/11 04:00
CRDT- 2002/12/10 04:00
PHST- 2002/12/10 04:00 [pubmed]
PHST- 2003/03/11 04:00 [medline]
PHST- 2002/12/10 04:00 [entrez]
AID - 10.2175/106143002x140233 [doi]
PST - ppublish
SO  - Water Environ Res. 2002 Sep-Oct;74(5):437-49. doi: 10.2175/106143002x140233.