PMID- 12470633
OWN - NLM
STAT- MEDLINE
DCOM- 20030124
LR  - 20220408
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 299
IP  - 5
DP  - 2002 Dec 20
TI  - Leucine promotes glucose uptake in skeletal muscles of rats.
PG  - 693-6
AB  - Soleus muscles isolated from normal rats were incubated to evaluate whether or 
      not leucine promotes glucose uptake under insulin-free conditions, using a 
      labeled 2-deoxyglucose uptake assay. Glucose uptake was promoted by 2mM leucine. 
      A metabolite of leucine, alpha-ketoisocaproic acid (alpha-KIC), also exhibited a 
      similar stimulatory effect, although this was not as potent as leucine. 
      Stimulation of glucose uptake by leucine was completely canceled by pre-treatment 
      with either 10 microM LY294002, a specific inhibitor of phosphatidylinositol 
      3-kinase (PI3-kinase), or 6 microM GF109203X, a specific inhibitor of protein 
      kinase C (PKC). No significant change was observed by pre-treatment with 1 microM 
      rapamycin, a specific inhibitor of mammalian target of rapamycin (mTOR). These 
      results suggest that leucine stimulates glucose transport in skeletal muscle via 
      PI3-kinase and PKC pathways independently of the mammalian target of mTOR. They 
      also suggest that leucine stimulates glucose transport by an insulin-independent 
      mechanism.
FAU - Nishitani, Shinobu
AU  - Nishitani S
AD  - Pharmaceutical Research Laboratories, Ajinomoto Co., Inc., 1-1, Suzuki-cho, 
      Kawasaki-ku, Kawasaki-shi, Kanagawa 210-8681, Japan. 
      shinobu_nishitani@ajinomoto.com
FAU - Matsumura, Tsuyoshi
AU  - Matsumura T
FAU - Fujitani, Shoji
AU  - Fujitani S
FAU - Sonaka, Ichiro
AU  - Sonaka I
FAU - Miura, Yutaka
AU  - Miura Y
FAU - Yagasaki, Kazumi
AU  - Yagasaki K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Insulin)
RN  - 0 (Keto Acids)
RN  - 816-66-0 (alpha-ketoisocaproic acid)
RN  - GMW67QNF9C (Leucine)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Culture Techniques
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - Glucose/*metabolism
MH  - Insulin/pharmacology
MH  - Keto Acids/pharmacology
MH  - Leucine/*pharmacology
MH  - Male
MH  - Models, Biological
MH  - Muscle, Skeletal/drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2002/12/10 04:00
MHDA- 2003/01/25 04:00
CRDT- 2002/12/10 04:00
PHST- 2002/12/10 04:00 [pubmed]
PHST- 2003/01/25 04:00 [medline]
PHST- 2002/12/10 04:00 [entrez]
AID - S0006291X02027171 [pii]
AID - 10.1016/s0006-291x(02)02717-1 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2002 Dec 20;299(5):693-6. doi: 
      10.1016/s0006-291x(02)02717-1.