PMID- 12472243
OWN - NLM
STAT- MEDLINE
DCOM- 20030103
LR  - 20111117
IS  - 0736-0266 (Print)
IS  - 0736-0266 (Linking)
VI  - 20
IP  - 6
DP  - 2002 Nov
TI  - Expression of monocyte chemoattractant protein-1 in primary cultures of rabbit 
      intervertebral disc cells.
PG  - 1298-304
AB  - Macrophages are considered essential for herniated disc resorption, and 
      chemokines may play a role in their recruitment. Here we demonstrate that 
      intervertebral disc cells are capable of producing monocyte chemoattractant 
      protein-1 (MCP-1), a CC chemokine that is chemotactic for macrophages. Nucleus 
      pulposus cells and anulus fibrosus cells were harvested from intervertebral discs 
      of healthy rabbits, and the cells were stimulated with either interleukin 
      (IL)-1beta or tumor necrosis factor (TNF)alpha. Reverse transcriptase polymerase 
      chain reaction demonstrated that IL-1beta and TNFalpha induced mRNA expression 
      for MCP-1 in nucleus pulposus and anulus fibrosus cells. Protein concentrations 
      of MCP-1 in the culture supernatants were quantitated by fluoroimmunoassay, which 
      showed that nucleus pulposus and anulus fibrosus cells dose- and time-dependently 
      produced MCP-1 after IL-1beta- and TNFalpha-stimulation, an event that was 
      completely abrogated by IL-1 receptor antagonist and anti-TNFalpha monoclonal 
      antibody, respectively. Nucleus pulposus cells produced significantly higher 
      levels of MCP-1 than did anulus fibrosus cells. Immunohistochemically, the 
      intensity of MCP-1 positive cells in nucleus pulposus cells was stronger than 
      that in anulus fibrosus cells. Altogether, our data clearly demonstrated the 
      production of MCP-1 in intervertebral disc cells, suggesting the possible 
      involvement of disc cells in an early stage of macrophage infiltration.
FAU - Yoshida, Masakazu
AU  - Yoshida M
AD  - Department of Orthopaedic Surgery, Kumamoto University School of Medicine, 1-1-1 
      Honjo, Kumamoto 860-8556, Japan. 995m9070@med.stud.kumamoto-u.ac.jp
FAU - Nakamura, Takafumi
AU  - Nakamura T
FAU - Kikuchi, Taro
AU  - Kikuchi T
FAU - Takagi, Katsumasa
AU  - Takagi K
FAU - Matsukawa, Akihiro
AU  - Matsukawa A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/pharmacology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics
MH  - Interleukin-1/pharmacology
MH  - Intervertebral Disc/*cytology/*physiology
MH  - Macrophages/cytology
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2002/12/11 04:00
MHDA- 2003/01/07 04:00
CRDT- 2002/12/11 04:00
PHST- 2002/12/11 04:00 [pubmed]
PHST- 2003/01/07 04:00 [medline]
PHST- 2002/12/11 04:00 [entrez]
AID - 10.1016/S0736-0266(02)00060-8 [doi]
PST - ppublish
SO  - J Orthop Res. 2002 Nov;20(6):1298-304. doi: 10.1016/S0736-0266(02)00060-8.