PMID- 12472279
OWN - NLM
STAT- MEDLINE
DCOM- 20021212
LR  - 20041117
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 118
IP  - 6
DP  - 2002 Dec
TI  - Anti-gp43 antibodies in the cerebrospinal fluid of patients with central nervous 
      system involvement by paracoccidioidomycosis.
PG  - 864-8
AB  - Paracoccidioidomycosis (PCM) is a chronic granulomatous infectious disease, 
      endemic in subtropical areas of Central and South America. The diagnosis of the 
      central nervous system (CNS) involvement with PCM (neuroparacoccidioidomycosis 
      [NPCM]) frequently is difficult. A definitive diagnosis usually is made by 
      visualization or isolation of Paracoccidioides brasiliensis from CNS biopsy or 
      necropsy material. In the present study, we determined the presence of anti-gp43 
      antibodies in the cerebrospinalfluid (CSF) of patients with CNS involvement in 
      PCM by enzyme-linked immunosorbent assay (ELISA) in 9 cases of NPCM and 15 
      control cases. ELISA anti-gp43 was compared with double immunodiffusion (DID). 
      ELISA anti-gp43 was positive in 8 (89%) of 9 CSF samples from patients with NPCM 
      and negative in all CSF samples of the control group. DID was negative in all CSF 
      samples from patients with NPCM and control samples. ELISA anti-gp43 in CSF 
      samples is better than DID for the diagnosis of NPCM. It is a sensitive and 
      specific diagnostic method and has high predictive values. To our knowledge, this 
      is thefirst time ELISA anti-gp43 was applied to CSF.
FAU - de Almeida, Sergio M
AU  - de Almeida SM
FAU - Queiroz-Telles, Flavio
AU  - Queiroz-Telles F
FAU - Doi, Elvira M
AU  - Doi EM
FAU - Ono, Margaret
AU  - Ono M
FAU - Werneck, Lineu C
AU  - Werneck LC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Antigens, Fungal)
RN  - 0 (Glycoproteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, Fungal/*immunology
MH  - Central Nervous System Fungal Infections/*cerebrospinal fluid/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glycoproteins/*immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*cerebrospinal fluid/immunology
EDAT- 2002/12/11 04:00
MHDA- 2002/12/13 04:00
CRDT- 2002/12/11 04:00
PHST- 2002/12/11 04:00 [pubmed]
PHST- 2002/12/13 04:00 [medline]
PHST- 2002/12/11 04:00 [entrez]
AID - 10.1309/H2LU-UX28-7QHN-V5H3 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2002 Dec;118(6):864-8. doi: 10.1309/H2LU-UX28-7QHN-V5H3.