PMID- 12472570
OWN - NLM
STAT- MEDLINE
DCOM- 20030123
LR  - 20191210
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 119
IP  - 4
DP  - 2002 Dec
TI  - A study on 289 consecutive Korean patients with acute leukaemias revealed 
      fluorescence in situ hybridization detects the MLL translocation without 
      cytogenetic evidence both initially and during follow-up.
PG  - 930-9
AB  - Translocations involving the MLL gene on the chromosome 11 (11q23) are frequently 
      observed in acute leukaemia. The detection of this genetic change has a unique 
      significance as a result of its implication of poor prognosis. To reveal the 
      utility of fluorescence in situ hybridization (FISH) in detecting the MLL 
      translocation, we analysed 289 consecutive Korean patients (children and adults) 
      with acute leukaemias using both conventional cytogenetic analysis (CC) and FISH, 
      placing an emphasis on the result discrepancies. Twenty-two of 289 patients 
      (7.6%) had the 11q23/MLL translocation. In nine of 22 patients (41%), only FISH 
      detected the translocation. In eight of these 22 patients, a total of 19 
      follow-up examinations were performed, of which FISH detected a significant level 
      of leukaemic cells harbouring the MLL translocation in five patients (26%) 
      without cytogenetic evidence. In addition to the MLL translocation, FISH detected 
      submicroscopic amplification, partial deletion of the MLL gene and trisomy 11 in 
      12 patients without cytogenetic evidence. In summary, up to 41% of the MLL 
      translocations at initial work-up and 26% during follow-up were detected by FISH 
      without cytogenetic evidence. Thus, we recommend that MLL FISH should be 
      performed in the diagnosis and monitoring of acute leukaemias in combination with 
      CC.
FAU - Kim, Hee Jin
AU  - Kim HJ
AD  - Department of Clinical Pathology, Department of Internal Medicine, and Cancer 
      Research Institute, Seoul National University College of Medicine, Seoul, Korea.
FAU - Cho, Han Ik
AU  - Cho HI
FAU - Kim, Eui Chong
AU  - Kim EC
FAU - Ko, Eun Kyong
AU  - Ko EK
FAU - See, Cha Ja
AU  - See CJ
FAU - Park, Seon Yang
AU  - Park SY
FAU - Lee, Dong Soon
AU  - Lee DS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
CIN - Br J Haematol. 2003 Jun;121(6):953-5. PMID: 12786810
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*genetics
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - Follow-Up Studies
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Karyotyping
MH  - Leukemia/diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - Neoplasm Proteins/genetics
MH  - *Proto-Oncogenes
MH  - *Transcription Factors
MH  - *Translocation, Genetic
EDAT- 2002/12/11 04:00
MHDA- 2003/01/24 04:00
CRDT- 2002/12/11 04:00
PHST- 2002/12/11 04:00 [pubmed]
PHST- 2003/01/24 04:00 [medline]
PHST- 2002/12/11 04:00 [entrez]
AID - 3937 [pii]
AID - 10.1046/j.1365-2141.2002.03937.x [doi]
PST - ppublish
SO  - Br J Haematol. 2002 Dec;119(4):930-9. doi: 10.1046/j.1365-2141.2002.03937.x.