PMID- 12472671
OWN - NLM
STAT- MEDLINE
DCOM- 20030110
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 56
IP  - 6
DP  - 2002 Dec
TI  - Analysis of gene expression during maturation of immature dendritic cells derived 
      from peripheral blood monocytes.
PG  - 593-601
AB  - Dendritic cells (DCs) are the most important antigen-presenting cells. Many 
      recent studies have compared the function of immature DCs (iDCs) and mature DCs 
      (mDCs), but there have been few reports of the molecular changes that occur in 
      DCs during maturation. Here, we report on differential gene expression in iDCs 
      generated from peripheral blood monocytes compared with mDCs. Gene expression was 
      evaluated using the differential display method after activation of iDCs with a 
      low concentration of lipopolysaccharide (LPS) to induce maturation. Proteasome 
      subunit alpha type 3 (PSMA3), transcription factor EC (TFEC) isoform and BTK 
      region clone 2f10-rpi were transiently upregulated. Tryptophanyl-tRNA synthetase 
      and CD63 antigen were upregulated for at least 24 h. Neuronal apoptosis 
      inhibitory protein (NAIP) and transforming growth factor-beta-induced 68 kDa 
      protein were downregulated. This is the first report of NAIP expression in human 
      DCs. By comparing the expression of NAIP with that of other members of the 
      inhibitor of apoptosis protein (IAP) family and the Bcl-2 family, only NAIP was 
      found to be strongly expressed in iDCs before stimulation by LPS. PSMA3 was also 
      induced in the DCs stimulated with immune complex. These findings might 
      contribute to our understanding of DC maturation and the effectiveness of 
      DC-based vaccines.
FAU - Matsunaga, T
AU  - Matsunaga T
AD  - First Department of Internal Medicine, Sapporo Medical University, Sapporo, 
      Japan.
FAU - Ishida, T
AU  - Ishida T
FAU - Takekawa, M
AU  - Takekawa M
FAU - Nishimura, S
AU  - Nishimura S
FAU - Adachi, M
AU  - Adachi M
FAU - Imai, K
AU  - Imai K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (NAIP protein, human)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuronal Apoptosis-Inhibitory Protein)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Apoptosis
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cysteine Endopeptidases/metabolism
MH  - Dendritic Cells/classification/drug effects/*immunology
MH  - Gene Expression Profiling
MH  - Humans
MH  - Immunophenotyping
MH  - Kinetics
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/*immunology
MH  - Multienzyme Complexes/metabolism
MH  - Nerve Tissue Proteins/biosynthesis/genetics
MH  - Neuronal Apoptosis-Inhibitory Protein
MH  - Polymerase Chain Reaction
MH  - Proteasome Endopeptidase Complex
MH  - Proto-Oncogene Proteins c-bcl-2/biosynthesis/genetics
MH  - RNA, Messenger/biosynthesis
MH  - Stem Cells/*immunology
MH  - Transforming Growth Factor beta/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2002/12/11 04:00
MHDA- 2003/01/11 04:00
CRDT- 2002/12/11 04:00
PHST- 2002/12/11 04:00 [pubmed]
PHST- 2003/01/11 04:00 [medline]
PHST- 2002/12/11 04:00 [entrez]
AID - 1179 [pii]
AID - 10.1046/j.1365-3083.2002.01179.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2002 Dec;56(6):593-601. doi: 10.1046/j.1365-3083.2002.01179.x.