PMID- 12472905 OWN - NLM STAT- MEDLINE DCOM- 20030116 LR - 20190630 IS - 0022-3042 (Print) IS - 0022-3042 (Linking) VI - 83 IP - 6 DP - 2002 Dec TI - Up-regulation of the neuronal serotoninergic phenotype in vitro: BDNF and cAMP share Trk B-dependent mechanisms. PG - 1525-8 AB - The effects of brain-derived neurotrophic factor (BDNF) and cAMP on the neuronal serotoninergic phenotype were studied in primary cultures of E14 rat embryonic rostral raphe. Short treatments (for 18 h) with BDNF or dibutyryl-cAMP induced an almost two-fold increase in the number of serotoninergic neurones and a dramatic extension and ramification of their neurites. These changes were associated with marked increases in the levels of mRNAs encoding the serotonin transporter, the 5-HT1A and 5-HT1B receptors and the BDNF receptor tyrosine kinase B (TrkB). Concomitant blockade of tyrosine kinases by genistein suppressed all the up-regulating effects of BDNF and cAMP on 5-hydroxytryptamine (5-HT) neurones. These findings suggest that an auto-amplifying mechanism underlies the promoting effect of BDNF on the differentiation of serotoninergic neurones through TrkB activation, which is also triggered by cAMP. FAU - Rumajogee, Prakasham AU - Rumajogee P AD - Laboratoire de Neurobiologie des Signaux Intercellulaires, CNRS UMR 7101, Universite Pierre et Marie Curie, Paris, France. FAU - Madeira, Alexandra AU - Madeira A FAU - Verge, Daniel AU - Verge D FAU - Hamon, Michel AU - Hamon M FAU - Miquel, Marie-Christine AU - Miquel MC LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Neurochem JT - Journal of neurochemistry JID - 2985190R RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Carrier Proteins) RN - 0 (Enzyme Inhibitors) RN - 0 (Membrane Glycoproteins) RN - 0 (Membrane Transport Proteins) RN - 0 (Nerve Tissue Proteins) RN - 0 (RNA, Messenger) RN - 0 (Receptor, Serotonin, 5-HT1B) RN - 0 (Receptors, Serotonin) RN - 0 (Receptors, Serotonin, 5-HT1) RN - 0 (Serotonin Plasma Membrane Transport Proteins) RN - 0 (Slc6a4 protein, rat) RN - 333DO1RDJY (Serotonin) RN - 63X7MBT2LQ (Bucladesine) RN - DH2M523P0H (Genistein) RN - E0399OZS9N (Cyclic AMP) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology MH - Bucladesine/pharmacology MH - Carrier Proteins/genetics/metabolism MH - Cell Differentiation/drug effects MH - Cells, Cultured MH - Cyclic AMP/*metabolism MH - Enzyme Inhibitors/pharmacology MH - Genistein/pharmacology MH - Membrane Glycoproteins/genetics/metabolism MH - *Membrane Transport Proteins MH - *Nerve Tissue Proteins MH - Neurites/drug effects MH - Neurons/cytology/drug effects/*metabolism MH - Phenotype MH - RNA, Messenger/metabolism MH - Raphe Nuclei/cytology MH - Rats MH - Rats, Wistar MH - Receptor, Serotonin, 5-HT1B MH - Receptor, trkB/genetics/*metabolism MH - Receptors, Serotonin/genetics/metabolism MH - Receptors, Serotonin, 5-HT1 MH - Serotonin/*metabolism MH - Serotonin Plasma Membrane Transport Proteins MH - Up-Regulation/drug effects/physiology EDAT- 2002/12/11 04:00 MHDA- 2003/01/17 04:00 CRDT- 2002/12/11 04:00 PHST- 2002/12/11 04:00 [pubmed] PHST- 2003/01/17 04:00 [medline] PHST- 2002/12/11 04:00 [entrez] AID - 1264 [pii] AID - 10.1046/j.1471-4159.2002.01264.x [doi] PST - ppublish SO - J Neurochem. 2002 Dec;83(6):1525-8. doi: 10.1046/j.1471-4159.2002.01264.x.