PMID- 12473384
OWN - NLM
STAT- MEDLINE
DCOM- 20030127
LR  - 20220309
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 65
IP  - 1
DP  - 2003 Jan 1
TI  - Evaluation of oxidative stress during apoptosis and necrosis caused by 
      D-galactosamine in rat liver.
PG  - 101-7
AB  - Eighteen and twenty-four hours after intraperitoneal administration of 
      D-galactosamine (1g/kg body weight) to rats, the activity of caspase-3-like 
      protease in the liver increased significantly compared with that in the control 
      group given saline. Histological examinations including the in situ terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method found 
      apoptotic hepatocytes 18 hr after the administration of D-galactosamine. 
      Caspase-3 activity was barely detectable in the plasma of control rats, but 
      increased significantly 24 hr after drug administration along with a dramatic 
      increase in glutamate-oxaloacetate transaminase (GOT). These results indicated 
      that D-galactosamine causes apoptosis in the liver by activating caspase-3, which 
      is released to the plasma by secondary necrosis. The concentration of lipid 
      hydroperoxides in the liver increased significantly 24 hr after D-galactosamine 
      administration. In contrast, the concentration of vitamin C in the liver 
      decreased significantly 18 and 24 hr after D-galactosamine administration. These 
      results suggest that D-galactosamine induces severe oxidative stress in the 
      liver, leading to extensive necrosis.
FAU - Sun, Fang
AU  - Sun F
AD  - Department of Food Science and Nutrition, Nara Women's University, Nara 630-8506, 
      Japan.
FAU - Hamagawa, Eri
AU  - Hamagawa E
FAU - Tsutsui, Chihiro
AU  - Tsutsui C
FAU - Sakaguchi, Naomi
AU  - Sakaguchi N
FAU - Kakuta, Yuri
AU  - Kakuta Y
FAU - Tokumaru, Sadako
AU  - Tokumaru S
FAU - Kojo, Shosuke
AU  - Kojo S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (Lipid Peroxides)
RN  - 1406-18-4 (Vitamin E)
RN  - 7535-00-4 (Galactosamine)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Ascorbic Acid/metabolism
MH  - Galactosamine/*pharmacology
MH  - Lipid Peroxides/metabolism
MH  - Liver/*drug effects/metabolism/pathology
MH  - Male
MH  - Necrosis
MH  - Oxidative Stress/*drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Vitamin E/metabolism
EDAT- 2002/12/11 04:00
MHDA- 2003/01/28 04:00
CRDT- 2002/12/11 04:00
PHST- 2002/12/11 04:00 [pubmed]
PHST- 2003/01/28 04:00 [medline]
PHST- 2002/12/11 04:00 [entrez]
AID - S000629520201420X [pii]
AID - 10.1016/s0006-2952(02)01420-x [doi]
PST - ppublish
SO  - Biochem Pharmacol. 2003 Jan 1;65(1):101-7. doi: 10.1016/s0006-2952(02)01420-x.