PMID- 12474304
OWN - NLM
STAT- MEDLINE
DCOM- 20030225
LR  - 20151008
IS  - 0029-0203 (Print)
IS  - 0029-0203 (Linking)
VI  - 106
IP  - 11
DP  - 2002 Nov
TI  - [Transdifferentiation of conjunctival epithelium after ocular surface 
      rehabilitation through deep lamellar sclerokeratoplasty].
PG  - 714-20
AB  - PURPOSE: The quality of the postoperative corneal epithelia of patients with 
      severe ocular surface disorders, whose ocular surface had been reconstructed 
      through deep lamellar sclerokeratoplasty (DLSKP) using allografts, was examined. 
      PATIENTS AND METHODS: Six eyes with ocular surface disorders in 6 patients who 
      had received DLSKP were observed for periods of 2 years or longer (average: 3 
      years and 10 months). The rehabilitated corneal epithelium cells of some of these 
      patients were analyzed with impression cytology and fluorescence in situ 
      hybridization (FISH) analysis methods. RESULTS: All 3 cases analyzed using the 
      impression cytology method showed normal corneal epithelium cell forms. In the 2 
      cases analyzed also with the FISH analysis method, in which the hosts and donors 
      were of the opposite gender, the cells were host-derived (99.3% and 98.8%). 
      CONCLUSION: It is considered that rehabilitation of severe ocular surface 
      disorders using allograft epithelial transplantation procedures, including 
      DL-SKP, is eventually concluded by transdifferentiation of the conjunctival 
      epithelium cells derived from the host.
FAU - Goto, Shin
AU  - Goto S
AD  - Department of Ophthalmology, Teikyo University School of Medicine, 2-11-1 Kaga, 
      Itabashi-ku, Tokyo 173-8605, Japan.
FAU - Sugata, Hirohisa
AU  - Sugata H
FAU - Kozakai, Mamoru
AU  - Kozakai M
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Nippon Ganka Gakkai Zasshi
JT  - Nippon Ganka Gakkai zasshi
JID - 7505716
SB  - IM
MH  - Adult
MH  - Conjunctiva/*cytology
MH  - Corneal Diseases/surgery
MH  - *Corneal Transplantation
MH  - Epithelial Cells/cytology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Sclera/*transplantation
MH  - Transplantation, Homologous
EDAT- 2002/12/12 04:00
MHDA- 2003/02/26 04:00
CRDT- 2002/12/12 04:00
PHST- 2002/12/12 04:00 [pubmed]
PHST- 2003/02/26 04:00 [medline]
PHST- 2002/12/12 04:00 [entrez]
PST - ppublish
SO  - Nippon Ganka Gakkai Zasshi. 2002 Nov;106(11):714-20.