PMID- 12474981
OWN - NLM
STAT- MEDLINE
DCOM- 20030512
LR  - 20170214
IS  - 1352-4585 (Print)
IS  - 1352-4585 (Linking)
VI  - 8
IP  - 6
DP  - 2002 Dec
TI  - TACE mRNA expression in peripheral mononudear cells precedes new lesions on MRI 
      in multiple sclerosis.
PG  - 447-51
AB  - Tumor necrosis factor-alpha (TNF-alpha) is involved in the pathogenesis of 
      multiple sclerosis (MS). It has to be released from its cell membrane-bound 
      precursor by proteolytic cleavage. This is mainly performed by a member of the 
      ADAM (a disintegrin and metalloproteinase) family of enzymes, 
      TNF-alpha-converting enzyme (TACE, ADAM 17). In a longitudinal study on 11 
      relapsing-remitting MS patients, we qualitatively determined mRNA expression of 
      TNF-alpha and TACE in peripheral blood mononuclear cells (PBMCs) without ex vivo 
      stimulation. mRNA expression was related to disease activity as assessed by 
      monthly gadolinium (Gd)-enhanced brain magnetic resonance imaging (MRI). Patients 
      found positive for TACE mRNA in PBMCs showed a significantly higher mean number 
      of new Gd-enhancing lesions per scan one month following PBMC sampling.
FAU - Seifert, T
AU  - Seifert T
AD  - Department of Neurology, Karl Franzens University, Auenbruggerplatz 22, Graz 
      A-8036, Austria. thomas.seifert@kfunigraz.ac.at
FAU - Kieseier, B C
AU  - Kieseier BC
FAU - Ropele, S
AU  - Ropele S
FAU - Strasser-Fuchs, S
AU  - Strasser-Fuchs S
FAU - Quehenberger, F
AU  - Quehenberger F
FAU - Fazekas, F
AU  - Fazekas F
FAU - Hartung, H P
AU  - Hartung HP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mult Scler
JT  - Multiple sclerosis (Houndmills, Basingstoke, England)
JID - 9509185
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - AU0V1LM3JT (Gadolinium)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.86 (ADAM17 Protein)
RN  - EC 3.4.24.86 (ADAM17 protein, human)
SB  - IM
MH  - ADAM Proteins
MH  - ADAM17 Protein
MH  - Adult
MH  - Blood-Brain Barrier/immunology
MH  - Brain/immunology/pathology
MH  - Enzyme Induction/immunology
MH  - Female
MH  - Gadolinium
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Leukocytes, Mononuclear/*enzymology
MH  - Longitudinal Studies
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Metalloendopeptidases/*genetics
MH  - Multiple Sclerosis, Relapsing-Remitting/immunology/*pathology
MH  - RNA, Messenger/analysis
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2002/12/12 04:00
MHDA- 2003/05/13 05:00
CRDT- 2002/12/12 04:00
PHST- 2002/12/12 04:00 [pubmed]
PHST- 2003/05/13 05:00 [medline]
PHST- 2002/12/12 04:00 [entrez]
AID - 10.1191/1352458502ms830oa [doi]
PST - ppublish
SO  - Mult Scler. 2002 Dec;8(6):447-51. doi: 10.1191/1352458502ms830oa.