PMID- 12475671
OWN - NLM
STAT- MEDLINE
DCOM- 20030116
LR  - 20190714
IS  - 1527-9995 (Electronic)
IS  - 0090-4295 (Linking)
VI  - 60
IP  - 6
DP  - 2002 Dec
TI  - Correlation between fluorescence in situ hybridization and testicular biopsy for 
      the prediction of spermatogenesis in 37 patients with nonobstructive azoospermia.
PG  - 1063-8
AB  - OBJECTIVES: We applied interphase fluorescence in situ hybridization (FISH) to 
      testis sections to examine the evidence of spermatogenesis in patients with 
      nonobstructive azoospermia. This technique was evaluated and compared with 
      conventional testicular histopathologic findings for the possibility of 
      additional clinical applications. METHODS: Thirty-seven consecutive patients with 
      nonobstructive azoospermia were carefully evaluated clinically. Testes were 
      biopsied for both sperm extraction and histopathologic examination. FISH staining 
      was performed with a CEP 18 SpectrumAqua/CEP X SpectrumGreen/CEP Y SpectrumOrange 
      probe. RESULTS: Eight of 11 cases (sensitivity 73%) that were found to have 
      spermatids on the histopathologic slides also were proven to produce haploid 
      cells by FISH staining. On the other hand, 21 of the 26 cases (specificity 81%) 
      for which no spermatids could be found on the histopathologic slides also had 
      only diploid cells by FISH staining. On the basis of the good correlation between 
      the FISH staining and conventional histopathologic findings, we could confirm the 
      diagnosis of spermatogenesis using both methods. CONCLUSIONS: FISH staining of 
      testicular sections allows more reliable prediction of spermatogenesis and 
      provides benefits for a patient's decision regarding fertility counseling.
FAU - Chiang, Han-Sun
AU  - Chiang HS
AD  - Department of Urology, Taipei Medical University Hospital, Taipei, Taiwan.
FAU - Yeh, Shauh-Der
AU  - Yeh SD
FAU - Lin, Wei-Min
AU  - Lin WM
FAU - Fang, Chia-Lang
AU  - Fang CL
FAU - Wei, Hsiao-Jui
AU  - Wei HJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Urology
JT  - Urology
JID - 0366151
SB  - IM
MH  - Biopsy
MH  - Confidence Intervals
MH  - Haploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Oligospermia/*pathology
MH  - Sensitivity and Specificity
MH  - *Spermatogenesis
MH  - Testis/*pathology
MH  - Tissue and Organ Harvesting/methods
EDAT- 2002/12/12 04:00
MHDA- 2003/01/17 04:00
CRDT- 2002/12/12 04:00
PHST- 2002/12/12 04:00 [pubmed]
PHST- 2003/01/17 04:00 [medline]
PHST- 2002/12/12 04:00 [entrez]
AID - S009042950201974X [pii]
AID - 10.1016/s0090-4295(02)01974-x [doi]
PST - ppublish
SO  - Urology. 2002 Dec;60(6):1063-8. doi: 10.1016/s0090-4295(02)01974-x.