PMID- 12477249
OWN - NLM
STAT- MEDLINE
DCOM- 20030116
LR  - 20190822
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 46
IP  - 10
DP  - 2002
TI  - Monitoring of active HHV-6 infection in bone marrow transplant recipients by real 
      time PCR; comparison to detection of viral DNA in plasma by qualitative PCR.
PG  - 701-5
AB  - Twelve (46%) of the 26 patients had human herpesvirus 6 (HHV-6) viremia after 
      bone marrow transplant (BMT). All isolates were recovered from the samples 
      obtained at 2 weeks after BMT. The sensitivity and the specificity of detection 
      of viral DNA in plasma by qualitative polymerase chain reaction (PCR) for 
      monitoring active virus replication were 92% and 97% respectively. Moreover, the 
      positive (85%) and negative (99%) predictive values were also high. The patients 
      with HHV-6 viremia showed a clear peak in HHV-6 DNA in peripheral blood 
      mononuclear cells (PBMCs) at 2 weeks after BMT, which was measured by real time 
      PCR. The virus DNA level in PBMCs between the two groups (patients with viremia 
      and patients without viremia) was statistically different at 2 weeks after BMT (P 
      = 0.033). In patients with HHV-6 viremia, mean HHV-6 DNA copy number was higher 
      in the samples collected at 2 weeks after BMT than the samples collected at any 
      other time period.
FAU - Ihira, Masaru
AU  - Ihira M
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi 470-1192, Japan.
FAU - Yoshikawa, Tetsushi
AU  - Yoshikawa T
FAU - Suzuki, Kyoko
AU  - Suzuki K
FAU - Ohashi, Masahiro
AU  - Ohashi M
FAU - Suga, Sadao
AU  - Suga S
FAU - Horibe, Keizo
AU  - Horibe K
FAU - Tanaka, Naoko
AU  - Tanaka N
FAU - Kimura, Hiroshi
AU  - Kimura H
FAU - Kojima, Seiji
AU  - Kojima S
FAU - Kato, Koji
AU  - Kato K
FAU - Matsuyama, Takaharu
AU  - Matsuyama T
FAU - Nishiyama, Yukihiro
AU  - Nishiyama Y
FAU - Asano, Yoshizo
AU  - Asano Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Bone Marrow Transplantation/*adverse effects
MH  - DNA, Viral/*blood/chemistry/genetics
MH  - Female
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - Immunocompromised Host
MH  - Male
MH  - Polymerase Chain Reaction/*methods
MH  - Predictive Value of Tests
MH  - Roseolovirus Infections/blood/*virology
MH  - Sensitivity and Specificity
MH  - Viremia/diagnosis
EDAT- 2002/12/13 04:00
MHDA- 2003/01/17 04:00
CRDT- 2002/12/13 04:00
PHST- 2002/12/13 04:00 [pubmed]
PHST- 2003/01/17 04:00 [medline]
PHST- 2002/12/13 04:00 [entrez]
AID - 10.1111/j.1348-0421.2002.tb02754.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 2002;46(10):701-5. doi: 10.1111/j.1348-0421.2002.tb02754.x.