PMID- 12477423
OWN - NLM
STAT- MEDLINE
DCOM- 20030714
LR  - 20210105
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 20 Suppl 4
DP  - 2002 Dec 19
TI  - A clinical grade cocktail of cytokines and PGE2 results in uniform maturation of 
      human monocyte-derived dendritic cells: implications for immunotherapy.
PG  - A8-A22
AB  - Dendritic cells (DCs) can induce tumor- or pathogen-specific T cell responses in 
      humans. We comprehensively compared the clinically available DC maturation 
      stimuli for their ability to promote uniformly mature DCs that elicit higher 
      levels of T cell responses. We compared the standard maturation stimulus, 
      autologous monocyte-conditioned medium (MCM), with a synthetic double stranded 
      RNA (poly I:C), soluble CD40 ligand trimer, and a defined cocktail of cytokines 
      (TNF-alpha, IL-1 beta, IL-6) and PGE(2) to promote mature phenotype and function 
      in human monocyte-derived DCs. The cocktail was the most efficient despite the 
      lack of induction of IL-12p70. While these results support the use of the 
      MCM-mimic cocktail in clinical DC immunotherapy trials, the roles of it's 
      individual constituents remain to be completely defined.
FAU - Lee, Andrew W
AU  - Lee AW
AD  - Laboratory of Cellular Physiology and Immunology, Rockefeller University, New 
      York, NY 10021, USA.
FAU - Truong, Tuan
AU  - Truong T
FAU - Bickham, Kara
AU  - Bickham K
FAU - Fonteneau, Jean-Francois
AU  - Fonteneau JF
FAU - Larsson, Marie
AU  - Larsson M
FAU - Da Silva, Ida
AU  - Da Silva I
FAU - Somersan, Selin
AU  - Somersan S
FAU - Thomas, Elaine K
AU  - Thomas EK
FAU - Bhardwaj, Nina
AU  - Bhardwaj N
LA  - eng
GR  - AI44628/AI/NIAID NIH HHS/United States
GR  - AI49958-01/AI/NIAID NIH HHS/United States
GR  - CA84512/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Cell Division/immunology
MH  - Culture Media, Conditioned
MH  - Cytokines/*pharmacology
MH  - Dendritic Cells/*cytology/metabolism
MH  - Dinoprostone/*pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - *Immunotherapy
MH  - Interferon-gamma/metabolism
MH  - Interleukin-12/metabolism
MH  - Lymphocyte Culture Test, Mixed
MH  - Monocytes/*cytology
EDAT- 2002/12/13 04:00
MHDA- 2003/07/15 05:00
CRDT- 2002/12/13 04:00
PHST- 2002/12/13 04:00 [pubmed]
PHST- 2003/07/15 05:00 [medline]
PHST- 2002/12/13 04:00 [entrez]
AID - S0264410X02003821 [pii]
AID - 10.1016/s0264-410x(02)00382-1 [doi]
PST - ppublish
SO  - Vaccine. 2002 Dec 19;20 Suppl 4:A8-A22. doi: 10.1016/s0264-410x(02)00382-1.