PMID- 12477649
OWN - NLM
STAT- MEDLINE
DCOM- 20030725
LR  - 20190826
IS  - 0302-2838 (Print)
IS  - 0302-2838 (Linking)
VI  - 42
IP  - 6
DP  - 2002 Dec
TI  - Clinical utility of a multiprobe FISH assay in voided urine specimens for the 
      detection of bladder cancer and its recurrences, compared with urinary cytology.
PG  - 547-52
AB  - OBJECTIVES: To evaluate the clinical utility of a Multi-color FISH (fluorescence 
      in situ hybridization) assay in voided urine specimens for the detection of 
      bladder cancer and its recurrences, comparing the results with those afforded by 
      urinary cytology. METHODS: Voided urine samples from 86 patients were obtained 
      for urine cytology and FISH analysis. The latter was performed using a mixture of 
      fluorescent labeled DNA probes for the centromeric regions of chromosomes 3, 7 
      and 17, and the 9p21 region. Cystoscopy with biopsy or tumor resection was 
      performed in all patients, comparing the pathological results with the 
      cytological and FISH findings. RESULTS: Urinary cytology affords an overall 
      sensitivity of 63.8%, the figure being 25% for grade 1, 66.6% for grade 2 and 
      94.7% for grade 3 tumors. The sensitivities for FISH were 53.3% for grade 1, 
      83.3% for grade 2 and 100% for grade 3 tumors, with an overall sensitivity of 
      80.4%. The specificities of urinary cytology and FISH were 86.1 and 85.3%, 
      respectively. CONCLUSIONS: FISH improves the sensitivity rates obtained with 
      urine cytology for bladder cancer detection in all tumor grades and stages, and 
      offers similar specificity. FISH doubles the accuracy of urinary cytology in 
      application to low grade-stage tumors, and detects all high grade infiltrating 
      tumors.
FAU - Placer, Jose
AU  - Placer J
AD  - Department of Urology, Hospital del Mar, IMAS, Passeig Maritim 25-29, 08003 
      Barcelona, Spain. 93165@imas.imim.es
FAU - Espinet, Blanca
AU  - Espinet B
FAU - Salido, Marta
AU  - Salido M
FAU - Sole, Francesc
AU  - Sole F
FAU - Gelabert-Mas, Antoni
AU  - Gelabert-Mas A
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Eur Urol
JT  - European urology
JID - 7512719
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/*urine
MH  - Sensitivity and Specificity
MH  - Urinary Bladder Neoplasms/*urine
MH  - Urine/cytology
EDAT- 2002/12/13 04:00
MHDA- 2003/07/26 05:00
CRDT- 2002/12/13 04:00
PHST- 2002/12/13 04:00 [pubmed]
PHST- 2003/07/26 05:00 [medline]
PHST- 2002/12/13 04:00 [entrez]
AID - S0302283802004487 [pii]
AID - 10.1016/s0302-2838(02)00448-7 [doi]
PST - ppublish
SO  - Eur Urol. 2002 Dec;42(6):547-52. doi: 10.1016/s0302-2838(02)00448-7.