PMID- 12478686
OWN - NLM
STAT- MEDLINE
DCOM- 20030211
LR  - 20061115
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 96
IP  - 6
DP  - 2002 Dec 25
TI  - DNA image cytometry and fluorescence in situ hybridization for noninvasive 
      detection of urothelial tumors in voided urine.
PG  - 374-9
AB  - BACKGROUND: Cystoscopy and histologic examination remain the standard methods for 
      initial tumor diagnosis and monitoring for early detection of recurrences, since 
      the sensitivity of conventional urinary cytology for the detection of urothelial 
      tumors in urinary specimens is low. DNA image cytometry (ICM) and fluorescence in 
      situ hybridization (FISH) have been suggested as ancillary tools. The goal of the 
      current study was to compare the diagnostic value of DNA image cytometry and FISH 
      for the noninvasive detection of urothelial tumors in voided urine. METHODS: 
      Cytospin preparations were prepared from voided urine collected prior to the 
      resection of 26 noninvasive (pTa) and 11 invasive (pT1-2) tumors. Specimens from 
      14 patients with benign prostatic hyperplasia were used as negative controls. DNA 
      ICM was performed using the AUTOCYTE trade mark cell analytical system on 
      Feulgen-stained cytospin specimens. The commercially available UroVysion trade 
      mark FISH multiprobe was used to analyze chromosomes 3, 7, and 17, and 9p21. 
      RESULTS: The overall sensitivity of cytology improved from 24% to 54% and to 78% 
      if supplemented by ICM or FISH, respectively. Image cytometry detected all 
      invasive tumors (pT1-2), while FISH missed one; FISH identified 19 of 26 (73%) 
      pTa tumors, while only 9 (35%) of these tumors were aneuploid by ICM. The results 
      of ICM and FISH were concordant in 37 of 51 (72%) cases. CONCLUSIONS: The current 
      study shows that both FISH and ICM can successfully be used as supplementary 
      methods to detect the clinically most relevant group of invasive bladder 
      carcinomas. However, UroVysion FISH is more sensitive in the detection of pTa 
      tumors than ICM, as it recognizes individual chromosomal alterations that 
      frequently prevail in urothelial tumors.
CI  - Copyright 2002 American Cancer Society.
FAU - Dalquen, Peter
AU  - Dalquen P
AD  - Institute for Pathology, University of Basel, Basel, Switzerland.
FAU - Kleiber, Brigitte
AU  - Kleiber B
FAU - Grilli, Bruno
AU  - Grilli B
FAU - Herzog, Michelle
AU  - Herzog M
FAU - Bubendorf, Lukas
AU  - Bubendorf L
FAU - Oberholzer, Martin
AU  - Oberholzer M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Carcinoma, Transitional Cell/*pathology/urine
MH  - DNA, Neoplasm/analysis
MH  - Humans
MH  - Image Cytometry/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Ploidies
MH  - Prostatic Hyperplasia/pathology/urine
MH  - Urinary Bladder Neoplasms/*pathology/urine
EDAT- 2002/12/13 04:00
MHDA- 2003/02/13 04:00
CRDT- 2002/12/13 04:00
PHST- 2002/12/13 04:00 [pubmed]
PHST- 2003/02/13 04:00 [medline]
PHST- 2002/12/13 04:00 [entrez]
AID - 10.1002/cncr.10881 [doi]
PST - ppublish
SO  - Cancer. 2002 Dec 25;96(6):374-9. doi: 10.1002/cncr.10881.