PMID- 12479572
OWN - NLM
STAT- MEDLINE
DCOM- 20030808
LR  - 20181113
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 239
IP  - 1-2
DP  - 2002 Oct
TI  - Analysis on the phenotype of E-FABP-gene knockout mice.
PG  - 83-6
AB  - The fatty acids are shown to be critical in the maintenance of the water 
      permeability barrier that is ascribed to the lipids in the intracellular milieu 
      of the cornified cell layer in the epidermis. In view of this importance in the 
      skin, we examined the phenotype of epidermal fatty acid binding protein 
      (E-FABP)-deficient mice. In spite of total lack of E-FABP expression in the 
      various tissues of E-FABP deficient mice, these animals appeared normal in gross 
      and histological examination. In Northern blot analysis for other FABPs, the gene 
      expression of heart (H-)-type FABP is specifically elevated in the liver of 
      neonatal heterozygous and homozygous mice, suggesting the functional compensation 
      of H-FABP for E-FABP deficiency during their development. In functional analyses 
      of the skin, the basal transepidermal water loss (TEWL) of the adult homozygous 
      mice showed lower levels compared with the wild-type mice, and the impairment of 
      recovery in TEWL was observed in the homozygous mice when the lipid barrier of 
      the skin was disrupted by acetone. These results demonstrate that E-FABP is 
      responsible for the water permeability barrier of the skin, although the 
      molecular mechanism remains to be further elucidated.
FAU - Owada, Yuji
AU  - Owada Y
AD  - Division of Histology, Department of Cell Biology, Graduate School of Medicine, 
      Tohoku University, Sendai, Japan.
FAU - Suzuki, Ichiro
AU  - Suzuki I
FAU - Noda, Tetsuo
AU  - Noda T
FAU - Kondo, Hisatake
AU  - Kondo H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Carrier Proteins)
RN  - 0 (Fabp5 protein, mouse)
RN  - 0 (Fabp7 protein, mouse)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Animals
MH  - Brain/cytology/metabolism
MH  - Carrier Proteins/genetics/*metabolism
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Fatty Acids/metabolism
MH  - Gene Targeting
MH  - Mice
MH  - Mice, Knockout
MH  - *Neoplasm Proteins
MH  - *Nerve Tissue Proteins
MH  - Phenotype
MH  - Protein Isoforms/genetics/*metabolism
EDAT- 2002/12/14 04:00
MHDA- 2003/08/09 05:00
CRDT- 2002/12/14 04:00
PHST- 2002/12/14 04:00 [pubmed]
PHST- 2003/08/09 05:00 [medline]
PHST- 2002/12/14 04:00 [entrez]
PST - ppublish
SO  - Mol Cell Biochem. 2002 Oct;239(1-2):83-6.