PMID- 12480182
OWN - NLM
STAT- MEDLINE
DCOM- 20030616
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 108
IP  - 1-2
DP  - 2002 Dec
TI  - Synaptotagmin I and IV are differentially regulated in the brain by the 
      recreational drug 3,4-methylenedioxymethamphetamine (MDMA).
PG  - 94-101
AB  - 3,4-Methylenedioxymethamphetamine (MDMA or Ecstasy) is a widely abused drug. In 
      brains of mice exposed to MDMA, we recently detected altered expression of 
      several cDNAs and genes by using the differential display polymerase chain 
      reaction (PCR) method. Expression of one such cDNA, which exhibited 98% sequence 
      homology with the synaptic vesicle protein synaptotagmin IV, decreased 2 h after 
      MDMA treatment. Herein, the effect of MDMA on expression of both synaptotagmin I 
      and IV was studied in detail, since the two proteins are functionally 
      interrelated. PCR analyses (semi-quantitative and real-time) confirmed that upon 
      treatment with MDMA, expression of synaptotagmin IV decreased both in the 
      midbrain and frontal cortex of mice. Decreases in the protein levels of 
      synaptotagmin IV were confirmed by Western immunoblotting with anti-synaptotagmin 
      IV antibodies. In contrast, the same exposure to MDMA increased expression of 
      synaptotagmin I in the midbrain, a region rich in serotonergic neurons, but not 
      in the frontal cortex. This differential expression was confirmed at the protein 
      level with anti-synaptotagmin I antibodies. MDMA did not induce down- or 
      up-regulation of synaptotagmin IV and I, respectively, in serotonin transporter 
      knockout mice (-/-) that are not sensitive to MDMA. Therefore, psychoactive 
      drugs, such as MDMA, appear to modulate expression of synaptic vesicle proteins, 
      and possibly vesicle trafficking, in the brain.
FAU - Peng, Weiping
AU  - Peng W
AD  - Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, 
      Israel.
FAU - Premkumar, Arumugam
AU  - Premkumar A
FAU - Mossner, Rainald
AU  - Mossner R
FAU - Fukuda, Mitsunori
AU  - Fukuda M
FAU - Lesch, K Peter
AU  - Lesch KP
FAU - Simantov, Rabi
AU  - Simantov R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Hallucinogens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SLC6A4 protein, human)
RN  - 0 (SYT1 protein, human)
RN  - 0 (SYT4 protein, human)
RN  - 0 (Serotonin Agents)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 0 (Slc6a4 protein, mouse)
RN  - 0 (Synaptotagmin I)
RN  - 0 (Syt1 protein, mouse)
RN  - 0 (Syt4 protein, mouse)
RN  - 134193-27-4 (Synaptotagmins)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/*metabolism
MH  - *Calcium-Binding Proteins
MH  - Carrier Proteins/genetics/metabolism
MH  - Down-Regulation/physiology
MH  - Hallucinogens
MH  - Humans
MH  - Male
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - *Membrane Transport Proteins
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Serotonin Agents/*pharmacology
MH  - Serotonin Plasma Membrane Transport Proteins
MH  - Synaptotagmin I
MH  - Synaptotagmins
EDAT- 2002/12/14 04:00
MHDA- 2003/06/17 05:00
CRDT- 2002/12/14 04:00
PHST- 2002/12/14 04:00 [pubmed]
PHST- 2003/06/17 05:00 [medline]
PHST- 2002/12/14 04:00 [entrez]
AID - S0169328X02005181 [pii]
AID - 10.1016/s0169-328x(02)00518-1 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2002 Dec;108(1-2):94-101. doi: 
      10.1016/s0169-328x(02)00518-1.