PMID- 12483023
OWN - NLM
STAT- MEDLINE
DCOM- 20030314
LR  - 20171101
IS  - 0030-3747 (Print)
IS  - 0030-3747 (Linking)
VI  - 34
IP  - 6
DP  - 2002 Nov-Dec
TI  - Changes in gene expression of ARPE-19 cells in response to vitreous treatment.
PG  - 357-65
AB  - PURPOSES: The purpose of this study is to determine the changes in gene 
      expression by a human retinal pigment epithelium (RPE) cell line (ARPE-19) in 
      response to vitreous treatment, which induces RPE proliferation and phenotypic 
      changes in vitro that mimic the repair response observed in vivo during 
      proliferative vitreoretinopathy. METHODS: ARPE-19 cells were grown for more than 
      4 weeks and their gene expression studied in: (1) subconfluent cultures treated 
      with 50% human vitreous for 72 h; (2) subconfluent cultures without vitreous 
      treatment, and (3) a confluent, nondividing monolayer. Total RNA was extracted 
      from RPE cells and differential gene expression between each condition was 
      determined using gene arrays (Clontech, Palo Alto, Calif., USA). Semiquantitative 
      RT-PCR was used to confirm the upregulation of 4 genes related to vitreous 
      treatment. In addition, the secretion of 1 of these upregulated gene products, 
      monocyte chemotactic protein 1 (MCP-1), was confirmed by ELISA. RESULTS: A 
      greater than threefold increase in the expression of mRNA for cell cycle 
      regulators, intracellular transducers, cell adhesion proteins, growth factors and 
      chemokines was observed following vitreous treatment of the RPE cell line and a 
      corresponding decrease in the expression of genes related to apoptosis. RT-PCR 
      confirmed the increased gene expression of MCP-1, intercellular adhesion 
      molecule-1, vascular cell adhesion protein 1 precursor and vascular endothelial 
      growth factor in vitreous-treated cells. Immunoassays further showed an increased 
      MCP-1 secretion by vitreous-treated RPE. CONCLUSIONS: These findings suggest that 
      in this in vitro vitreous treatment model, ARPE-19 cells participate in a mock 
      repair response by upregulating genes encoding for proteins associated with 
      inflammation and wound healing.
CI  - Copyright 2002 S. Karger AG, Basel
FAU - Fan, Wei
AU  - Fan W
AD  - Department of Ophthalmology & Visual Sciences, Kentucky Lions Eye Center, 
      University of Louisville School of Medicine, Louisville, KY 40202, USA.
FAU - Zheng, Jing Juan
AU  - Zheng JJ
FAU - Peiper, Stephen C
AU  - Peiper SC
FAU - McLaughlin, Barbara J
AU  - McLaughlin BJ
LA  - eng
GR  - EY 02853/EY/NEI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Ophthalmic Res
JT  - Ophthalmic research
JID - 0267442
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA Primers)
RN  - 0 (Eye Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - DNA Primers/chemistry
MH  - Down-Regulation
MH  - Eye Proteins/physiology
MH  - Gene Expression/*physiology
MH  - Gene Expression Profiling
MH  - Humans
MH  - Pigment Epithelium of Eye/*metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Up-Regulation
MH  - Vitreous Body/*physiology
EDAT- 2002/12/17 04:00
MHDA- 2003/03/15 04:00
CRDT- 2002/12/17 04:00
PHST- 2002/12/17 04:00 [pubmed]
PHST- 2003/03/15 04:00 [medline]
PHST- 2002/12/17 04:00 [entrez]
AID - 67048 [pii]
AID - 10.1159/000067048 [doi]
PST - ppublish
SO  - Ophthalmic Res. 2002 Nov-Dec;34(6):357-65. doi: 10.1159/000067048.