PMID- 12484570
OWN - NLM
STAT- MEDLINE
DCOM- 20030114
LR  - 20201208
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 61
IP  - 12
DP  - 2002 Dec
TI  - High-throughput molecular profiling of high-grade astrocytomas: the utility of 
      fluorescence in situ hybridization on tissue microarrays (TMA-FISH).
PG  - 1078-84
AB  - Due to recent biological and technical advances, the list of potentially useful 
      candidate genes is rapidly expanding in the study of brain tumors. However, 
      traditional methods of screening individual genes in individual samples are slow 
      and tedious, often with consumption of precious resources after only a few 
      experiments. This study evaluates the feasibility of high-throughput molecular 
      analysis using fluorescence in situ hybridization (FISH) on glioma tissue 
      microarrays (TMA). A single microarray paraffin block was constructed using 65 
      WHO grade III and IV astrocytomas, sampled in duplicate with 0.6-mm-diameter 
      punch cores. FISH was used to detect common alterations, such as EGFR 
      amplification, chromosome 7, 9, and 10 aneusomies and deletions of 1p, 19q, PTEN, 
      DMBT1, and p16. Of 585 hybridization sets, 508 (87%) yielded interpretable data, 
      with hybridization failure in 33 (5.5%) and dislodged tissue in 44 sets (7.5%), 
      respectively. Glioblastomas harbored significantly more alterations than 
      anaplastic astrocytomas, with the overall frequencies of alterations similar to 
      those reported using other techniques. The overall concordance rate between 
      paired tumor core samples was 93%. We conclude that TMA-FISH is an efficient and 
      reliable method for detecting molecular alterations in high-grade astrocytomas.
FAU - Fuller, Christine E
AU  - Fuller CE
AD  - Division of Neuropathology, Barnes-Jewish Hospital, Washington University Medical 
      Center, St. Louis, Missouri 63110, USA.
FAU - Wang, Huamin
AU  - Wang H
FAU - Zhang, Wei
AU  - Zhang W
FAU - Fuller, Gregory N
AU  - Fuller GN
FAU - Perry, Arie
AU  - Perry A
LA  - eng
GR  - CA55261/CA/NCI NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
RN  - 0 (Agglutinins)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DMBT1 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - *Agglutinins
MH  - Astrocytoma/*genetics/metabolism/pathology
MH  - Brain Neoplasms/*genetics/metabolism/pathology
MH  - Calcium-Binding Proteins
MH  - Chromosome Deletion
MH  - Chromosomes, Human/genetics
MH  - DNA-Binding Proteins
MH  - ErbB Receptors/genetics/metabolism
MH  - Gene Deletion
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Neoplasm Proteins/genetics/metabolism
MH  - PTEN Phosphohydrolase
MH  - Phosphoric Monoester Hydrolases/genetics/metabolism
MH  - Protein Array Analysis/*methods
MH  - Receptors, Cell Surface/genetics/metabolism
MH  - Tumor Suppressor Proteins/genetics/metabolism
EDAT- 2002/12/18 04:00
MHDA- 2003/01/15 04:00
CRDT- 2002/12/18 04:00
PHST- 2002/12/18 04:00 [pubmed]
PHST- 2003/01/15 04:00 [medline]
PHST- 2002/12/18 04:00 [entrez]
AID - 10.1093/jnen/61.12.1078 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 2002 Dec;61(12):1078-84. doi: 10.1093/jnen/61.12.1078.