PMID- 12486094 OWN - NLM STAT- MEDLINE DCOM- 20030130 LR - 20170214 IS - 0022-1554 (Print) IS - 0022-1554 (Linking) VI - 50 IP - 12 DP - 2002 Dec TI - Suspension (S)-FISH, a new technique for interphase nuclei. PG - 1697-8 AB - We describe a versatile method for performing fluorescence in situ hybridization (FISH) in suspension instead of on a slide as usually done. This so-called suspension-FISH (S-FISH) opens new possibilities for the analysis of shape and functions of the human interphase nucleus. The procedure is described and the first results using this approach are presented. FAU - Steinhaeuser, Ulf AU - Steinhaeuser U AD - Institute of Human Genetics, Jena, Germany. FAU - Starke, Heike AU - Starke H FAU - Nietzel, Angela AU - Nietzel A FAU - Lindenau, Joerg AU - Lindenau J FAU - Ullmann, Peter AU - Ullmann P FAU - Claussen, Uwe AU - Claussen U FAU - Liehr, Thomas AU - Liehr T LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Histochem Cytochem JT - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society JID - 9815334 RN - 0 (Suspensions) SB - IM MH - Cell Nucleus/*metabolism MH - Humans MH - In Situ Hybridization, Fluorescence/*methods MH - *Interphase MH - Suspensions EDAT- 2002/12/18 04:00 MHDA- 2003/01/31 04:00 CRDT- 2002/12/18 04:00 PHST- 2002/12/18 04:00 [pubmed] PHST- 2003/01/31 04:00 [medline] PHST- 2002/12/18 04:00 [entrez] AID - 10.1177/002215540205001216 [doi] PST - ppublish SO - J Histochem Cytochem. 2002 Dec;50(12):1697-8. doi: 10.1177/002215540205001216.