PMID- 1248665
OWN - NLM
STAT- MEDLINE
DCOM- 19760419
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 4
IP  - 2
DP  - 1976 Jan
TI  - Failure to observe testosterone-induced nucleus-lysosome interaction in rat 
      ventral prostate.
PG  - 115-29
AB  - The possibility that sex steroids act to promote the association of lysosomes 
      with nuclei was studied by 2 methods in the secretory epithelium of ventral 
      prostate of castrated rats treated with androgens: (a) electron microscopic (EM) 
      examination of intact tissue and (b) study of fresh nuclear suspensions isolated 
      from prostate homogenates using the fluorescing dye acridine orange (AO) and EM. 
      AO-stained particles in nuclear suspensions were found to correspond to either 
      (i) section granules, (ii) primary lysosomes or (iii) heterogenous dense bodies 
      (HDB), considered to be lysosomes. Intact prostate tissue and crude nuclear 
      suspensions were studied in adult rats 3-21 days after castration and in normal 
      rats. Changes in nuclear architecture are evident 8 days or more after 
      castration; the number of HDB increase while secretion granules and primary 
      lysosomes decrease. Whether from prostate of castrated, normal or hormone-treated 
      animals, a small number of the lysosomal HDB or primary lysosomes are seen 
      closely apposed to epithelial cell nuclei. In response to short-term testosterone 
      administration (15 or 60 min) in 10- and 21-day castrate rats, chromatin 
      distribution appears to become more condensed; yet the number of associations of 
      nuclei with lysosomal elements does not change. These studies of androgen action 
      in rat ventral prostate (a classical target organ for the study of androgen 
      action) provide no evidence to support the idea that lysosomal association with, 
      or invasion of, nuclei in target cells is a general feature of sex steroid 
      hormone action.
FAU - Sepsenwol, S
AU  - Sepsenwol S
FAU - Hechter, O
AU  - Hechter O
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Acridines)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Acridines
MH  - Animals
MH  - Binding Sites
MH  - Castration
MH  - Cell Nucleus/drug effects/*ultrastructure
MH  - Epithelial Cells
MH  - Epithelium/drug effects/ultrastructure
MH  - Lysosomes/drug effects/*ultrastructure
MH  - Male
MH  - Microscopy, Electron
MH  - Microscopy, Fluorescence
MH  - Prostate/drug effects/*ultrastructure
MH  - Rats
MH  - Testosterone/*pharmacology
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
AID - 0303-7207(76)90031-9 [pii]
AID - 10.1016/0303-7207(76)90031-9 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1976 Jan;4(2):115-29. doi: 10.1016/0303-7207(76)90031-9.