PMID- 12488352
OWN - NLM
STAT- MEDLINE
DCOM- 20030117
LR  - 20161124
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 144
IP  - 1
DP  - 2003 Jan
TI  - Dehydroepiandrosterone down-regulates the expression of peroxisome 
      proliferator-activated receptor gamma in adipocytes.
PG  - 253-9
AB  - Dehydroepiandrosterone (DHEA) is expected to have a weight-reducing effect. In 
      this study, we evaluated the effect of DHEA on genetically obese Otsuka Long 
      Evans Fatty rats (OLETF) compared with Long-Evans Tokushima rats (LETO) as 
      control. Feeding with 0.4% DHEA-containing food for 2 wk reduced the weight of 
      sc, epididymal, and perirenal adipose tissue in association with decreased plasma 
      leptin levels in OLETF. Adipose tissue from OLETF showed increased expression of 
      peroxisome proliferator-activated receptor gamma (PPARgamma) protein, which was 
      prevented by DHEA treatment. Further, we examined the effect of DHEA on PPARgamma 
      in primary cultured adipocytes and monolayer adipocytes differentiated from rat 
      preadipocytes. PPARgamma protein level was decreased in a time- and 
      concentration-dependent manner, and DHEA significantly reduced mRNA levels of 
      PPARgamma, adipocyte lipid-binding protein, and sterol regulatory element-binding 
      protein, but not CCAAT/enhancer binding protein alpha. DHEA-sulfate also reduced 
      the PPARgamma protein, but dexamethasone, testosterone, or androstenedione did 
      not alter its expression. In addition, treatment with DHEA for 5 d reduced the 
      triglyceride content in monolayer adipocytes. These results suggest that DHEA 
      down-regulates adiposity through the reduction of PPARgamma in adipocytes.
FAU - Kajita, Kazuo
AU  - Kajita K
AD  - The Third Department of Internal Medicine, Gifu University School of Medicine, 
      Tsukasa-machi 40, Gifu 500-8705, Japan.
FAU - Ishizuka, Tatsuo
AU  - Ishizuka T
FAU - Mune, Tomoatsu
AU  - Mune T
FAU - Miura, Atsushi
AU  - Miura A
FAU - Ishizawa, Masayoshi
AU  - Ishizawa M
FAU - Kanoh, Yoshinori
AU  - Kanoh Y
FAU - Kawai, Yasunori
AU  - Kawai Y
FAU - Natsume, Yoshiyuki
AU  - Natsume Y
FAU - Yasuda, Keigo
AU  - Yasuda K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fabp7 protein, rat)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Leptin)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Srebf1 protein, rat)
RN  - 0 (Sterol Regulatory Element Binding Protein 1)
RN  - 0 (Transcription Factors)
RN  - 0 (Triglycerides)
RN  - 459AG36T1B (Dehydroepiandrosterone)
RN  - 57B09Q7FJR (Dehydroepiandrosterone Sulfate)
SB  - IM
MH  - Adipocytes/chemistry/*metabolism
MH  - Animals
MH  - CCAAT-Enhancer-Binding Protein-alpha/genetics
MH  - CCAAT-Enhancer-Binding Proteins/genetics
MH  - Carrier Proteins/genetics
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/genetics
MH  - Dehydroepiandrosterone/administration & dosage/*pharmacology
MH  - Dehydroepiandrosterone Sulfate/pharmacology
MH  - Diet
MH  - Dose-Response Relationship, Drug
MH  - Epididymis
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Gene Expression Regulation/*drug effects
MH  - Kinetics
MH  - Leptin/blood
MH  - Male
MH  - *Neoplasm Proteins
MH  - *Nerve Tissue Proteins
MH  - Obesity/*metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Inbred OLETF
MH  - Rats, Long-Evans
MH  - Receptors, Cytoplasmic and Nuclear/analysis/*genetics
MH  - Sterol Regulatory Element Binding Protein 1
MH  - Transcription Factors/analysis/*genetics
MH  - Triglycerides/analysis
EDAT- 2002/12/19 04:00
MHDA- 2003/01/18 04:00
CRDT- 2002/12/19 04:00
PHST- 2002/12/19 04:00 [pubmed]
PHST- 2003/01/18 04:00 [medline]
PHST- 2002/12/19 04:00 [entrez]
AID - 10.1210/en.2002-220039 [doi]
PST - ppublish
SO  - Endocrinology. 2003 Jan;144(1):253-9. doi: 10.1210/en.2002-220039.