PMID- 12488502
OWN - NLM
STAT- MEDLINE
DCOM- 20030328
LR  - 20091119
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 72
IP  - 6
DP  - 2002 Dec
TI  - Interleukin-10 differently regulates monocyte chemoattractant protein-1 gene 
      expression depending on the environment in a human monoblastic cell line, UG3.
PG  - 1198-205
AB  - Interleukin (IL)-4, IL-10, and IL-13 affect monocyte/macrophage functions 
      including regulation of cytokine production. We analyzed the regulatory effects 
      of these cytokines on cytokine production using a human monoblastic cell line, 
      UG3. It is interesting that IL-10 up-regulated, whereas IL-4 and IL-13 
      down-regulated monocyte chemoattractant protein-1 (MCP-1) production by 
      unstimulated UG3 cells. IL-10-induced expression of MCP-1 mRNA occurred without 
      de novo protein synthesis at transcriptional and post-transcriptional levels. The 
      enhancement of binding activity of nuclear factor Sp1 (Sp-1) and signal 
      transducer and activators of transcription (STAT)1 and 3 but not nuclear factor 
      kappaB (NF-kappaB) was associated with this IL-10-induced MCP-1 expression. 
      Furthermore, IL-10 suppressed lipopolysaccharide (LPS)-induced NF-kappaB binding 
      but not Sp-1. The present results suggest IL-10 has two contrasting actions on 
      the MCP-1 production of monocytes/macrophages, between the resting and activated 
      conditions. The combination of activated Sp-1 and STATs is important for 
      IL-10-induced MCP-1 expression in resting monocytes/macrophages, and the 
      inhibition of LPS-induced NF-kappaB binding is crucial for down-regulation of 
      MCP-1 by IL-10 in stimulated monocytes/macrophages.
FAU - Ikeda, Takashi
AU  - Ikeda T
AD  - Third Department of Internal Medicine, National Defense Medical College, Saitama, 
      Japan.
FAU - Sato, Ken
AU  - Sato K
FAU - Kuwada, Naruo
AU  - Kuwada N
FAU - Matsumura, Takuya
AU  - Matsumura T
FAU - Yamashita, Takuya
AU  - Yamashita T
FAU - Kimura, Fumihiko
AU  - Kimura F
FAU - Hatake, Kiyohiko
AU  - Hatake K
FAU - Ikeda, Kazuma
AU  - Ikeda K
FAU - Motoyoshi, Kazuo
AU  - Motoyoshi K
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interleukin-13)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (STAT1 protein, human)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Trans-Activators)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/*genetics
MH  - DNA-Binding Proteins/drug effects
MH  - Feedback, Physiological
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Interleukin-10/*pharmacology
MH  - Interleukin-13/pharmacology
MH  - Interleukin-4/pharmacology
MH  - NF-kappa B/drug effects
MH  - RNA, Messenger/drug effects
MH  - STAT1 Transcription Factor
MH  - STAT3 Transcription Factor
MH  - Sp1 Transcription Factor/drug effects
MH  - Trans-Activators/drug effects
EDAT- 2002/12/19 04:00
MHDA- 2003/03/29 05:00
CRDT- 2002/12/19 04:00
PHST- 2002/12/19 04:00 [pubmed]
PHST- 2003/03/29 05:00 [medline]
PHST- 2002/12/19 04:00 [entrez]
PST - ppublish
SO  - J Leukoc Biol. 2002 Dec;72(6):1198-205.