PMID- 12488579
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 1110-7251 (Electronic)
IS  - 1110-7243 (Print)
IS  - 1110-7243 (Linking)
VI  - 2
IP  - 3
DP  - 2002
TI  - Randomly Amplified DNA Fingerprinting: A Culmination of DNA Marker Technologies 
      Based on Arbitrarily-Primed PCR Amplification.
PG  - 141-150
AB  - Arbitrarily-primed DNA markers can be very useful for genetic fingerprinting and 
      for facilitating positional cloning of genes. This class of technologies is 
      particularly important for less studied species, for which genome sequence 
      information is generally not known. The technologies include Randomly Amplified 
      Polymorphic DNA (RAPD), DNA Amplification Fingerprinting (DAF), and Amplified 
      Fragment Length Polymorphism (AFLP). We have modified the DAF protocol to produce 
      a robust PCR-based DNA marker technology called Randomly Amplified DNA 
      Fingerprinting (RAF). While the protocol most closely resembles DAF, it is much 
      more robust and sensitive because amplicons are labelled with either radioactive 
      33P or fluorescence in a 30-cycle PCR, and then separated and detected on large 
      polyacrylamide sequencing gels. Highly reproducible RAF markers were readily 
      amplified from either purified DNA or alkali-treated intact leaf tissue. RAF 
      markers typically display dominant inheritance. However, a small but significant 
      portion of the RAF markers exhibit codominant inheritance and represent 
      microsatellite loci. RAF compares favorably with AFLP for efficiency and 
      reliability on many plant genomes, including the very large and complex genomes 
      of sugarcane and wheat. While the two technologies detect about the same number 
      of markers per large polyacrylamide gel, advantages of RAF over AFLP include: (i) 
      no requirement for enzymatic template preparation, (ii) one instead of two PCRs, 
      and (iii) overall cost. RAF and AFLP were shown to differ in the selective basis 
      of amplification of markers from genomes and could therefore be used in 
      complementary fashion for some genetic studies.
FAU - Waldron, Julie
AU  - Waldron J
FAU - Peace, Cameron P
AU  - Peace CP
FAU - Searle, Iain R
AU  - Searle IR
FAU - Furtado, Agnelo
AU  - Furtado A
FAU - Wade, Nick
AU  - Wade N
FAU - Findlay, Ian
AU  - Findlay I
FAU - Graham, Michael W
AU  - Graham MW
FAU - Carroll, Bernard J
AU  - Carroll BJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biomed Biotechnol
JT  - Journal of biomedicine & biotechnology
JID - 101135740
PMC - PMC161367
EDAT- 2002/12/19 04:00
MHDA- 2002/12/19 04:00
PMCR- 2002/01/01
CRDT- 2002/12/19 04:00
PHST- 2002/12/19 04:00 [pubmed]
PHST- 2002/12/19 04:00 [medline]
PHST- 2002/12/19 04:00 [entrez]
PHST- 2002/01/01 00:00 [pmc-release]
AID - S1110724302206026 [pii]
AID - 10.1155/S1110724302206026 [doi]
PST - ppublish
SO  - J Biomed Biotechnol. 2002;2(3):141-150. doi: 10.1155/S1110724302206026.