PMID- 12494468
OWN - NLM
STAT- MEDLINE
DCOM- 20030206
LR  - 20160303
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 103
IP  - 5
DP  - 2003 Feb 20
TI  - Clustering of deletions on chromosome 13 in benign and low-malignant lipomatous 
      tumors.
PG  - 616-23
AB  - Deletions and structural rearrangements of the long arm of chromosome 13 are 
      frequently observed in benign and low-malignant lipomatous tumors, but nothing is 
      known about their molecular genetic consequences. We assessed the karyotypes of 
      40 new and 22 previously published cases (35 ordinary lipomas, 15 spindle 
      cell/pleomorphic lipomas, 2 myxolipomas, 1 angiomyxolipoma and 9 atypical 
      lipomatous tumors) with chromosome 13-abnormalities, and found bands 13q12-22 to 
      be frequently affected. Twenty-seven cases with structural abnormalities within 
      this region were selected for breakpoint and deletion mapping by metaphase 
      fluorescence in situ hybridization (FISH), using a set of 20 probes. Deletions 
      were found in 23 of 27 cases. The remaining 4 cases had seemingly balanced 
      rearrangements. The breakpoints were scattered but clustered to band 13q14, and 
      in all cases with unbalanced abnormalities, a limited region within band 13q14 
      was partially or completely deleted. A deletion within band 13q14 was found 
      together with a breakpoint on the other homologue in 5 cases, 4 of which could be 
      tested further with regard to the status of the retinoblastoma (RB1)-gene. In all 
      4 cases, only 1 copy of the gene was deleted. In addition to the breaks and 
      deletions in the vicinity of the RB1-locus, several other regions of 13q were 
      recurrently affected, e.g., in the vicinity of the hereditary breast cancer 
      (BRCA2; 13q12)- and lipoma HMGIC fusion partner (LHFP; 13q13)- genes. Our 
      findings strongly indicate that deletion of a limited region (approximately 2.5 
      Mbp) within 13q14, distal to the RB1-locus, is of importance in the development 
      of a subset of lipomatous tumors.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Dahlen, Anna
AU  - Dahlen A
AD  - Department of Clinical Genetics, University Hospital, Lund, Sweden. 
      anna.dahlen@klingen.lu.se
FAU - Debiec-Rychter, Maria
AU  - Debiec-Rychter M
FAU - Pedeutour, Florence
AU  - Pedeutour F
FAU - Domanski, Henryk A
AU  - Domanski HA
FAU - Hoglund, Mattias
AU  - Hoglund M
FAU - Bauer, Henrik C F
AU  - Bauer HC
FAU - Rydholm, Anders
AU  - Rydholm A
FAU - Sciot, Raf
AU  - Sciot R
FAU - Mandahl, Nils
AU  - Mandahl N
FAU - Mertens, Fredrik
AU  - Mertens F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Angiolipoma/*genetics/pathology
MH  - *Chromosome Deletion
MH  - Chromosomes, Artificial, Yeast/genetics
MH  - Chromosomes, Human, Pair 13/*genetics
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lipoma/classification/*genetics/pathology
MH  - Liposarcoma/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Translocation, Genetic
RF  - 34
EDAT- 2002/12/21 04:00
MHDA- 2003/02/07 04:00
CRDT- 2002/12/21 04:00
PHST- 2002/12/21 04:00 [pubmed]
PHST- 2003/02/07 04:00 [medline]
PHST- 2002/12/21 04:00 [entrez]
AID - 10.1002/ijc.10864 [doi]
PST - ppublish
SO  - Int J Cancer. 2003 Feb 20;103(5):616-23. doi: 10.1002/ijc.10864.