PMID- 1249880
OWN - NLM
STAT- MEDLINE
DCOM- 19760429
LR  - 20131121
IS  - 0021-7263 (Print)
IS  - 0021-7263 (Linking)
VI  - 138
IP  - 2
DP  - 1976 Feb
TI  - Immunological reactions involving leukocytes: I. Detection of antibodies to human 
      granulocytes by measurement of the metabolic events associated with phagocytosis.
PG  - 43-7
AB  - During phagocytosis, glucose oxidation via the hexose monophosphate shunt (HMS) 
      is markedly stimulated in human polymorphonuclear leukocytes. A method is 
      described for the detection of antibodies to human granulocytes which exploits 
      this fact. The source of antibodies was rabbit antihuman leukocyte serum (RAHLS) 
      obtained by repeated immunization of normal rabbits with human granulocyte 
      homogenates. HMS activity was quantitated by measuring the release of 14CO2 from 
      glucose-1-14C. Under precisely defined experimental conditions, RAHLS and its IgG 
      fraction inhibited 14CO2 release. The inhibitory effect of RAHLS on human 
      granulocyte phagocytosis-associated HMS activity could be reduced by prior 
      absorption of RAHLS with human granulocytes. Compared to the cytotoxic and 
      leukoagglutination tests, this method appears more sensitive with respect to 
      detection of xenogeneic antibodies to human granulocytes.
FAU - Laleli, Y R
AU  - Laleli YR
FAU - Bilezikian, S B
AU  - Bilezikian SB
FAU - Tsan, M F
AU  - Tsan MF
FAU - Hodkinson, B A
AU  - Hodkinson BA
FAU - McIntyre, P A
AU  - McIntyre PA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Johns Hopkins Med J
JT  - The Johns Hopkins medical journal
JID - 0072456
RN  - 0 (Antibodies)
RN  - 0 (Antilymphocyte Serum)
RN  - 142M471B3J (Carbon Dioxide)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Absorption
MH  - *Antibodies
MH  - Antilymphocyte Serum/pharmacology
MH  - Carbon Dioxide/metabolism
MH  - Cytotoxicity Tests, Immunologic
MH  - Depression, Chemical
MH  - Glucose/metabolism
MH  - Granulocytes/*immunology/metabolism/physiology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Leukocytes/*immunology
MH  - *Phagocytosis
EDAT- 1976/02/01 00:00
MHDA- 1976/02/01 00:01
CRDT- 1976/02/01 00:00
PHST- 1976/02/01 00:00 [pubmed]
PHST- 1976/02/01 00:01 [medline]
PHST- 1976/02/01 00:00 [entrez]
PST - ppublish
SO  - Johns Hopkins Med J. 1976 Feb;138(2):43-7.