PMID- 12498810
OWN - NLM
STAT- MEDLINE
DCOM- 20030203
LR  - 20191106
IS  - 1521-6616 (Print)
IS  - 1521-6616 (Linking)
VI  - 105
IP  - 3
DP  - 2002 Dec
TI  - Dysfunctional and short-lived subsets in monocyte-derived dendritic cells from 
      patients with advanced cancer.
PG  - 286-95
AB  - Dendritic cells (DCs) are antigen-presenting cells specialized for the induction 
      of the primary T-cell response. Tumor immunotherapy using DCs loaded with tumor 
      antigens is under way for patients with several types of advanced malignancies. 
      In this study, DC-like cells (Mo-DCs) were generated from peripheral blood 
      monocytes with granulocyte-macrophage colony-stimulating factor and 
      interleukin-4. The antigen-presenting abilities, including capture of apoptotic 
      tumor cells, IL-12 secretion, expression of antigen-presentation-related 
      molecules (HLA-ABC, HLA-DR, and CD80), and mixed leukocyte reaction, of Mo-DCs 
      from 37 patients with advanced cancer (pMo-DCs) were compared to those of 20 
      healthy volunteers (hMo-DCs). Seven days after the initial culture, no 
      significant difference was found in either the number or the size of 
      Mo-DC-forming colonies between the two groups. However, most of the 
      antigen-presenting abilities of pMo-DCs were weaker than those of hMo-DCs on day 
      7. On day 14, both number and size of colonies were significantly decreased in 
      pMo-DCs but not in hMo-DCs. Interestingly, the antigen-presenting abilities of 
      the remaining pMo-DCs gradually strengthened with time and by day 14 no 
      significant difference was observed between pMo-DCs and hMo-DCs. These results 
      indicate that pMo-DCs contain dysfunctional and short-lived Mo-DC subsets.
FAU - Onishi, Hideya
AU  - Onishi H
AD  - Department of Cancer Therapy and Research, Graduate School of Medical Sciences, 
      Kyushu University, Fukuoka, 812-8582, Japan.
FAU - Morisaki, Takashi
AU  - Morisaki T
FAU - Baba, Eishi
AU  - Baba E
FAU - Kuga, Hirotaka
AU  - Kuga H
FAU - Kuroki, Hideo
AU  - Kuroki H
FAU - Matsumoto, Kotaro
AU  - Matsumoto K
FAU - Tanaka, Masao
AU  - Tanaka M
FAU - Katano, Mitsuo
AU  - Katano M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 187348-17-0 (Interleukin-12)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Aged
MH  - *Antigen Presentation
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interleukin-12/metabolism
MH  - Lymphocyte Activation
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - Middle Aged
MH  - Monocytes/cytology
MH  - Neoplasms/*therapy
MH  - Time Factors
EDAT- 2002/12/25 04:00
MHDA- 2003/02/04 04:00
CRDT- 2002/12/25 04:00
PHST- 2002/12/25 04:00 [pubmed]
PHST- 2003/02/04 04:00 [medline]
PHST- 2002/12/25 04:00 [entrez]
AID - S1521661602952939 [pii]
AID - 10.1006/clim.2002.5293 [doi]
PST - ppublish
SO  - Clin Immunol. 2002 Dec;105(3):286-95. doi: 10.1006/clim.2002.5293.