PMID- 12499670
OWN - NLM
STAT- MEDLINE
DCOM- 20030423
LR  - 20220321
IS  - 0289-0003 (Print)
IS  - 0289-0003 (Linking)
VI  - 19
IP  - 11
DP  - 2002 Nov
TI  - Cultivation and characterization of planarian neuronal cells isolated by 
      fluorescence activated cell sorting (FACS).
PG  - 1257-65
AB  - Studies using molecular markers have revealed that planarians possess a highly 
      organized brain. Here we separated brain neurons from dissociated planarian head 
      cells by fluorescence activated cell sorting (FACS), and characterized them by 
      single cell PCR analysis and cell culture. Dissociated cells were labeled with 
      three different fluorescent dyes, Hoechst 33258, Merocyanine 540, and Propidium 
      Iodide (PI), and fractioned by FACS. Interestingly, we have succeeded in 
      identifying a cell fraction specific to the head, which we have named the 
      head-abundant cell fraction (HAC). Most of the HAC expressed neuron-specific 
      genes and proteins. When they were cultured in vitro, they showed an ability to 
      extend neurites on several types of extracellular matrices (ECMs), and, depending 
      on the ECM type used, presented a high level of plasticity in morphology and gene 
      expression.
FAU - Asami, Maki
AU  - Asami M
AD  - Department of Biofunctional Chemistry, Faculty of Biomolecular Science, Okayama 
      University Graduate School of Natural Science and Technology, Okayama, Japan.
FAU - Nakatsuka, Tsuyoshi
AU  - Nakatsuka T
FAU - Hayashi, Tetsutaro
AU  - Hayashi T
FAU - Kou, Kenji
AU  - Kou K
FAU - Kagawa, Hiroaki
AU  - Kagawa H
FAU - Agata, Kiyokazu
AU  - Agata K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Zoolog Sci
JT  - Zoological science
JID - 8702287
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Animals
MH  - Brain/cytology
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - *Flow Cytometry
MH  - Fluorescent Dyes
MH  - Gene Expression Regulation
MH  - Head
MH  - Neurons/*cytology/*metabolism
MH  - Organ Specificity
MH  - Planarians/anatomy & histology/*cytology
MH  - Polymerase Chain Reaction
EDAT- 2002/12/25 04:00
MHDA- 2003/04/24 05:00
CRDT- 2002/12/25 04:00
PHST- 2002/12/25 04:00 [pubmed]
PHST- 2003/04/24 05:00 [medline]
PHST- 2002/12/25 04:00 [entrez]
AID - 10.2108/zsj.19.1257 [doi]
PST - ppublish
SO  - Zoolog Sci. 2002 Nov;19(11):1257-65. doi: 10.2108/zsj.19.1257.