PMID- 12499678
OWN - NLM
STAT- MEDLINE
DCOM- 20030528
LR  - 20190605
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 64
IP  - 11
DP  - 2002 Nov
TI  - Leader-body junction sequence of the viral subgenomic mRNAs of porcine 
      reproductive and respiratory syndrome virus isolated in Taiwan.
PG  - 961-5
AB  - In combination of utilizing a leader specific primer and primers complementary to 
      porcine reproductive and respiratory syndrome virus (PRRSV) genome through 
      RT-PCR, the leader junction sequences of subgenomic mRNA (sg mRNA) was identified 
      from a Taiwanese isolate of PRRSV. Thirty-six cDNA clones derived from sg mRNAs 
      2, 3, 4, 5, 6 and 7 were determined. The junction sequences analyzed from 
      different sg mRNA were found to contain a similar 5 nucleotide sequence motif, 
      (U/G)(C/A)(A/G)CC. The distance between the junction site and the translation 
      initiation codon of the down stream open reading frame varied from 4 to 226 
      nucleotides. Minor heterogenecity was observed in the nucleotide sequence 
      surrounding the junctions from all six sg mRNA analyzed. However, for sg mRNA 7, 
      two junction sites approximately 103 nucleotides apart from each other were 
      identified. The additional site is a new junction not previously reported in sg 
      mRNA 7 from other PRRSV strains.
FAU - Lin, Yu-Chieh
AU  - Lin YC
AD  - Department of Veterinary Medicine, National Taiwan University, Taipei.
FAU - Chang, Ruey-Yi
AU  - Chang RY
FAU - Chueh, Ling-Ling
AU  - Chueh LL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Spliced Leader)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - *Genome, Viral
MH  - Open Reading Frames/genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - RNA, Messenger/genetics
MH  - RNA, Spliced Leader/*genetics
MH  - RNA, Viral/*genetics
MH  - Sequence Alignment
MH  - Taiwan
EDAT- 2002/12/25 04:00
MHDA- 2003/05/29 05:00
CRDT- 2002/12/25 04:00
PHST- 2002/12/25 04:00 [pubmed]
PHST- 2003/05/29 05:00 [medline]
PHST- 2002/12/25 04:00 [entrez]
AID - 10.1292/jvms.64.961 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2002 Nov;64(11):961-5. doi: 10.1292/jvms.64.961.