PMID- 12502873
OWN - NLM
STAT- MEDLINE
DCOM- 20030122
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 77
IP  - 2
DP  - 2003 Jan
TI  - Proline 78 is crucial for human immunodeficiency virus type 1 Nef to 
      down-regulate class I human leukocyte antigen.
PG  - 1589-94
AB  - Human immunodeficiency virus type 1 Nef down-regulates human leukocyte antigen 
      class I (HLA-I) in T lymphocytes, and the down-regulation involves the Nef 
      proline-rich domain (PRD) containing four prolines at positions 69, 72, 75, and 
      78. We used a Sendai virus vector with nef and examined regulation by Nef of 
      HLA-I and CD4 in suspension cultures of cells such as T lymphocytes. Analyses of 
      a series of PRD substitution mutants indicated that, because the substitution of 
      Pro78 with Ala abolished down-regulation of HLA-I but not of CD4, Pro78 is 
      important for HLA-I down-regulation in T lymphocytes.
FAU - Yamada, Takeshi
AU  - Yamada T
AD  - Division of Infectious Diseases, Advanced Clinical Research Center, The 
      University of Tokyo, Japan.
FAU - Kaji, Naotoshi
AU  - Kaji N
FAU - Odawara, Takashi
AU  - Odawara T
FAU - Chiba, Joe
AU  - Chiba J
FAU - Iwamoto, Aikichi
AU  - Iwamoto A
FAU - Kitamura, Yoshihiro
AU  - Kitamura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Gene Products, nef)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (nef Gene Products, Human Immunodeficiency Virus)
RN  - 9DLQ4CIU6V (Proline)
SB  - IM
MH  - Cell Line
MH  - Down-Regulation/*physiology
MH  - Gene Products, nef/chemistry/*physiology
MH  - HIV-1/*physiology
MH  - Histocompatibility Antigens Class I/*physiology
MH  - Humans
MH  - Proline/*physiology
MH  - T-Lymphocytes/immunology
MH  - nef Gene Products, Human Immunodeficiency Virus
PMC - PMC140770
EDAT- 2002/12/28 04:00
MHDA- 2003/01/23 04:00
PMCR- 2003/01/01
CRDT- 2002/12/28 04:00
PHST- 2002/12/28 04:00 [pubmed]
PHST- 2003/01/23 04:00 [medline]
PHST- 2002/12/28 04:00 [entrez]
PHST- 2003/01/01 00:00 [pmc-release]
AID - 0767 [pii]
AID - 10.1128/jvi.77.2.1589-1594.2003 [doi]
PST - ppublish
SO  - J Virol. 2003 Jan;77(2):1589-94. doi: 10.1128/jvi.77.2.1589-1594.2003.