PMID- 12502896
OWN - NLM
STAT- MEDLINE
DCOM- 20030701
LR  - 20191106
IS  - 0914-3505 (Print)
IS  - 0914-3505 (Linking)
VI  - 42
IP  - 3
DP  - 2002 Sep
TI  - An examination of different fetal specific antibodies and magnetic activated cell 
      sorting for the enrichment of fetal erythroblasts from maternal blood.
PG  - 175-80
AB  - The aim of the present study was to compare the rates of fetal cells obtained 
      after separation from maternal blood by magnetic activated cell sorting (MACS) 
      using different fetal specific antibodies, and to evaluate the potential role of 
      this method in the prenatal diagnosis of fetal trisomies. Peripheral blood 
      samples were obtained from 42 women carrying chromosomally normal fetuses and 
      from 4 women with aneuploid fetuses (2 cases of 47,XX,+18 and 2 of 47,XY,+21) at 
      9-20 weeks of gestation. After fetal cells were enriched by MACS with three 
      different monoclonal antibodies (GPA, CD71, CD14), fluorescence in situ 
      hybridization (FISH) with chromosome X, and Y-specific probes was performed to 
      detect the rates of fetal cells in the samples sorted. FISH with chromosome 13-, 
      18-, and 21-specific probes was carried out to compare proportions of cells with 
      three-signal nuclei in chromosomally normal and abnormal groups. In male infants, 
      X- and Y-positive cells were detected in 80%, 73.3%, and 66.6% of samples after 
      the separation by antibodies CD14, GPA, and CD71, respectively. The percentage of 
      nuclei with three signals was increased in pregnancies with trisomy, ranging 
      between 2% and 5.18%. Pregnancies with normal fetuses showed 0 to 3.7% of nuclei 
      with three signals. The data demonstrate that fetal cell detection varies 
      depending on the antibodies used for cell sorting. This study provides further 
      evidence on the feasibility of screening for fetal chromosomal abnormalities by 
      enriching maternal blood for fetal cells and using FISH.
FAU - Zhao, Xiao Xi
AU  - Zhao XX
AD  - Department of Obstetrics and Gynecology, Nagoya City University Medical School, 
      Nagoya, Aichi 467-8601, Japan. zhaoxx@med.nagoya-cu.ac.jp
FAU - Ozaki, Yasuhiko
AU  - Ozaki Y
FAU - Suzumori, Nobuhiro
AU  - Suzumori N
FAU - Sato, Takeshi
AU  - Sato T
FAU - Suzumori, Kaoru
AU  - Suzumori K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Congenit Anom (Kyoto)
JT  - Congenital anomalies
JID - 9306292
SB  - IM
MH  - Aneuploidy
MH  - Chromosomes, Human/immunology
MH  - *Erythroblasts
MH  - Female
MH  - Fetus/cytology/*immunology
MH  - *Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - Prenatal Diagnosis/methods
EDAT- 2002/12/28 04:00
MHDA- 2003/07/02 05:00
CRDT- 2002/12/28 04:00
PHST- 2002/12/28 04:00 [pubmed]
PHST- 2003/07/02 05:00 [medline]
PHST- 2002/12/28 04:00 [entrez]
AID - 10.1111/j.1741-4520.2002.tb00890.x [doi]
PST - ppublish
SO  - Congenit Anom (Kyoto). 2002 Sep;42(3):175-80. doi: 
      10.1111/j.1741-4520.2002.tb00890.x.