PMID- 12502982
OWN - NLM
STAT- MEDLINE
DCOM- 20030121
LR  - 20190628
IS  - 0003-3022 (Print)
IS  - 0003-3022 (Linking)
VI  - 98
IP  - 1
DP  - 2003 Jan
TI  - Halogenated anesthetics reduce interleukin-1beta-induced cytokine secretion by 
      rat alveolar type II cells in primary culture.
PG  - 74-81
AB  - BACKGROUND: Alveolar epithelial type II (AT II ) cells participate in the 
      intraalveolar cytokine network by secreting cytokines and are widely exposed to 
      volatile anesthetics during general anesthesia. The aim of the current study was 
      to evaluate the effects of halothane, enflurane, and isoflurane on rat AT II cell 
      cytokine secretions in AT II primary cell cultures. METHODS: Alveolar epithelial 
      type II primary cell cultures were obtained from adult rat lungs. AT II cells 
      were stimulated by recombinant murine interleukin-1beta (rmIL-1beta) to mimic an 
      inflammatory response, and immediately exposed for various duration to different 
      concentration of halothane, enflurane, or isoflurane. Interleukin-6, macrophage 
      inflammatory protein-2 (MIP-2), and monocyte chemoattractant protein-1 (MCP-1) 
      protein concentrations were then measured in cell culture supernatants. 
      Recombinant mIL-1beta-stimulated AT II cells exposed to air served as control. 
      RESULTS: Halothane, isoflurane, and enflurane (1 minimum alveolar concentration 
      [MAC], 4 h) decreased rmIL-1beta-stimulated AT II cell secretions of 
      interleukin-6, MIP-2, and MCP-1, but did not modify total protein secretion. 
      Halothane exposure decreased rmIL-1beta-stimulated AT II cell secretions of 
      interleukin-6, MIP-2, and MCP-1 in a dose- and time-dependent manner. Total 
      protein concentrations remained unchanged except AT II 1.5 MAC of halothane, and 
      no cytotoxic effect could be evidenced by lactate dehydrogenase release. These 
      effects were transient as rmIL-1beta-stimulated AT II cell secretions of 
      interleukin-6 and MIP-2 progressively reached control values between 4 and 24 h 
      after the end of halothane exposure. However, MCP-1 inhibition persisted until 24 
      h. rmIL-1beta-induced MIP-2 and tumor necrosis factor-alpha mRNA expression were 
      decreased by 36 and 24%, respectively, after halothane exposure. CONCLUSIONS: The 
      current study shows that exposure of rmIL-1beta-stimulated AT II cells to 
      volatile anesthetics reversibly alters their cytokine secretion. Therefore, 
      volatile anesthesia, by modulating pulmonary epithelial cell secretion of 
      inflammatory cytokines, might affect the lung inflammatory response.
FAU - Giraud, Olivier
AU  - Giraud O
AD  - Institut National de la Sante Et de la Recherche Medicale, Unite 408, Faculte de 
      Medecine Xavier Bichat, Departement d'Anesthesie-Reanimation Chirurgicale, Centre 
      Hospitalo-Universitaire Bichat Claude Bernard, Paris, France. giraud@igr.fr
FAU - Molliex, Serge
AU  - Molliex S
FAU - Rolland, Corinne
AU  - Rolland C
FAU - Lecon-Malas, Veronique
AU  - Lecon-Malas V
FAU - Desmonts, Jean-Marie
AU  - Desmonts JM
FAU - Aubier, Michel
AU  - Aubier M
FAU - Dehoux, Monique
AU  - Dehoux M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anesthesiology
JT  - Anesthesiology
JID - 1300217
RN  - 0 (Anesthetics, Inhalation)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 91I69L5AY5 (Enflurane)
RN  - CYS9AKD70P (Isoflurane)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - UQT9G45D1P (Halothane)
SB  - IM
CIN - Anesthesiology. 2003 Jan;98(1):3-4. PMID: 12502970
MH  - Anesthetics, Inhalation/*pharmacology
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Cytokines/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enflurane/pharmacology
MH  - Halothane/pharmacology
MH  - Interleukin-1/*antagonists & inhibitors/*pharmacology
MH  - Interleukin-6/biosynthesis
MH  - Isoflurane/pharmacology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Pneumonia/metabolism/pathology
MH  - Protein Biosynthesis
MH  - Pulmonary Alveoli/cytology/drug effects/*metabolism
MH  - Rats
MH  - Recombinant Proteins/pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 2002/12/28 04:00
MHDA- 2003/01/22 04:00
CRDT- 2002/12/28 04:00
PHST- 2002/12/28 04:00 [pubmed]
PHST- 2003/01/22 04:00 [medline]
PHST- 2002/12/28 04:00 [entrez]
AID - 00000542-200301000-00015 [pii]
AID - 10.1097/00000542-200301000-00015 [doi]
PST - ppublish
SO  - Anesthesiology. 2003 Jan;98(1):74-81. doi: 10.1097/00000542-200301000-00015.