PMID- 12503878
OWN - NLM
STAT- MEDLINE
DCOM- 20030519
LR  - 20061115
IS  - 1058-8108 (Print)
IS  - 1058-8108 (Linking)
VI  - 11
IP  - 4
DP  - 2002 Dec
TI  - L-amino acid oxidase from Trimeresurus jerdonii snake venom: purification, 
      characterization, platelet aggregation-inducing and antibacterial effects.
PG  - 345-52
AB  - An L-amino acid oxidase (LAO), designated as TJ-LAO, was purified to homogeneity 
      from the venom of Trimeresurus jerdonii by Sephadex G-100 and Q Sepharose HP 
      chromatography. The molecular weight of this enzyme was 110 kD as estimated by 
      analytical gel filtration and was 55 kD by SDS-polyacrylamide gel 
      electrophoresis, suggesting that the enzyme is composed of two subunits. The 
      enzyme has an absorption spectrum characteristic of flavoproteins, containing 2 
      moles of FMN per mole of enzyme. The N-terminal sequence of TJ-LAO shares high 
      homology with other viperid snake venom LAOs. Homology with elapid venom LAO is 
      lower. TJ-LAO inhibited the growth of Escherichia coli, Staphylococcus aureus, 
      Pseudomonas aeruginosa, and Bacillus megaterium. The antibacterial effect 
      associated with LAO activity was elminated with the addition of catalase. 
      Platelets in platelet-rich plasma aggregated upon the addition of TJ-LAO. The 
      enzyme-induced aggregation was inhibited by catalase, suggesting formation of 
      H2O2 was essential for TJ-LAO to induce platelet aggregation. These results 
      showed H2O2 formation is important for the biological effects of LAO.
FAU - Lu, Qiu-Min
AU  - Lu QM
AD  - Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of 
      Sciences, Kunming 650223, Yunnan, China.
FAU - Wei, Qin
AU  - Wei Q
FAU - Jin, Yang
AU  - Jin Y
FAU - Wei, Ji-Fu
AU  - Wei JF
FAU - Wang, Wan-Yu
AU  - Wang WY
FAU - Xiong, Yu-Liang
AU  - Xiong YL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Nat Toxins
JT  - Journal of natural toxins
JID - 9208016
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Crotalid Venoms)
RN  - 0 (Trimeresurus venoms)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Amino Acid Oxidoreductases/chemistry/*isolation & purification/pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anti-Bacterial Agents/chemistry/*isolation & purification/pharmacology
MH  - Crotalid Venoms/*enzymology
MH  - Gram-Negative Bacteria/*drug effects
MH  - Gram-Positive Bacteria/*drug effects
MH  - L-Amino Acid Oxidase
MH  - Molecular Sequence Data
MH  - Platelet Aggregation/drug effects
MH  - Rabbits
MH  - Sequence Homology, Amino Acid
EDAT- 2002/12/31 04:00
MHDA- 2003/05/20 05:00
CRDT- 2002/12/31 04:00
PHST- 2002/12/31 04:00 [pubmed]
PHST- 2003/05/20 05:00 [medline]
PHST- 2002/12/31 04:00 [entrez]
PST - ppublish
SO  - J Nat Toxins. 2002 Dec;11(4):345-52.