PMID- 12504600
OWN - NLM
STAT- MEDLINE
DCOM- 20030321
LR  - 20191106
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 21
IP  - 4
DP  - 2002 Dec
TI  - Noxious stimulation induces Trk receptor and downstream ERK phosphorylation in 
      spinal dorsal horn.
PG  - 684-95
AB  - Several lines of evidence suggest that the brain-derived neurotrophic factor 
      (BDNF) acts as central pain neuromodulator. We examined the ability of different 
      types of peripheral stimulation to activate the BDNF high-affinity receptor, 
      TrkB, in the spinal cord. We found that noxious chemical, mechanical, or thermal 
      stimuli, but not innocuous stimuli, caused Trk phosphorylation in the spinal 
      cord. These changes were rapid and transient and restricted to somatotopically 
      appropriate spinal segments. We observed, both in vitro and in vivo, that 
      exogenous BDNF induced a rapid activation of ERK, a signaling kinase important in 
      the development of acute pain. Finally, we found that sequestering BDNF in vivo 
      with a TrkB-IgG fusion molecule significantly reduced the activation of ERK 
      evoked by noxious stimulation. These data suggest that BDNF, once released with 
      activity from primary afferent nociceptors, exerts a neuromodulatory role in pain 
      processing through stimulation of postsynaptic TrkB receptors and subsequent 
      activation of ERK.
FAU - Pezet, Sophie
AU  - Pezet S
AD  - Centre for Neuroscience Research, Guy's King's and St. Thomas' School of 
      Biomedical Sciences, King's College London, London SE1, United Kingdom. 
      sophiepezet@kcl.ac.uk
FAU - Malcangio, Marzia
AU  - Malcangio M
FAU - Lever, Isobel J
AU  - Lever IJ
FAU - Perkinton, Michael S
AU  - Perkinton MS
FAU - Thompson, Stephen W N
AU  - Thompson SW
FAU - Williams, Robert J
AU  - Williams RJ
FAU - McMahon, Stephen B
AU  - McMahon SB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Oils)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - TYY1MA9BSY (mustard oil)
SB  - IM
MH  - Afferent Pathways/drug effects/*metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Male
MH  - Mitogen-Activated Protein Kinases/drug effects/*metabolism
MH  - Mustard Plant
MH  - Nerve Fibers, Unmyelinated/drug effects/metabolism
MH  - Nociceptors/drug effects/*metabolism
MH  - Pain/chemically induced/*metabolism/physiopathology
MH  - Pain Measurement
MH  - Phosphorylation
MH  - Physical Stimulation
MH  - Plant Extracts
MH  - Plant Oils
MH  - Posterior Horn Cells/drug effects/*metabolism
MH  - Precipitin Tests
MH  - Presynaptic Terminals/drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/drug effects/*metabolism
MH  - Signal Transduction/drug effects/physiology
MH  - Synaptic Transmission/drug effects/physiology
EDAT- 2002/12/31 04:00
MHDA- 2003/03/22 04:00
CRDT- 2002/12/31 04:00
PHST- 2002/12/31 04:00 [pubmed]
PHST- 2003/03/22 04:00 [medline]
PHST- 2002/12/31 04:00 [entrez]
AID - S1044743102912054 [pii]
AID - 10.1006/mcne.2002.1205 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2002 Dec;21(4):684-95. doi: 10.1006/mcne.2002.1205.