PMID- 12505251
OWN - NLM
STAT- MEDLINE
DCOM- 20030115
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 138
IP  - 2
DP  - 2002 Oct 15
TI  - Molecular cytogenetic aberrations in CD30+ anaplastic large cell lymphoma cell 
      lines.
PG  - 95-101
AB  - Anaplastic large cell lymphomas (ALCL) in children represent a heterogeneous 
      group of neoplasms with regard to the cell lineages involved. The chromosomal 
      5q35 breakpoint (bp) and the expression of the NPM/ALK fusion gene are the most 
      remarkable molecular cytogenetic features of these malignancies. To identify new 
      locations of ALCL-related oncogenes, comparative genomic hybridization (CGH) was 
      applied to three ALCL cell lines (SU-DHL-1, Karpas 299, and DEL) exhibiting the 
      5q35 bp and expressing the NPM/ALK transcript. The CGH profiles were compared 
      with those obtained with DNA from U937, HL-60 cells, and altered lymph nodes from 
      two children with ALCL. Significant DNA copy number gains and/or losses were 
      observed on several chromosomes in all ALCL cell lines. Distinct amplicons were 
      detected on 1q21 approximately q44 (DEL), 7q12 (SU-DHL-1), and 1q12 approximately 
      q22 (Karpas 299) regions. The NPM/ALK fusion gene was confirmed by fluorescence 
      in situ hybridization (FISH) analysis in more than 80% of interphase nuclei and 
      metaphase spreads. Enhanced expression of TGF-beta2 and c-MET candidate genes 
      located at the amplified regions was revealed in DEL and SU-DHL1 cell lines by 
      Northern blot analysis. These findings delineate chromosomal imbalances in 
      ALCL-derived cell lines in parallel with high level of amplification covering 
      target DNA sequences, which could play a role in ALCL pathogenesis.
FAU - Gogusev, Jean
AU  - Gogusev J
AD  - INSERM U507, Hopital Necker, Paris, France. gogusev@necker.fr
FAU - Telvi, Louise
AU  - Telvi L
FAU - Nezelof, Christian
AU  - Nezelof C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Ki-1 Antigen)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Ki-1 Antigen/*analysis
MH  - Lymphoma, Large B-Cell, Diffuse/*genetics
MH  - Nucleic Acid Hybridization
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 2002/12/31 04:00
MHDA- 2003/01/16 04:00
CRDT- 2002/12/31 04:00
PHST- 2002/12/31 04:00 [pubmed]
PHST- 2003/01/16 04:00 [medline]
PHST- 2002/12/31 04:00 [entrez]
AID - S0165460802005897 [pii]
AID - 10.1016/s0165-4608(02)00589-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Oct 15;138(2):95-101. doi: 
      10.1016/s0165-4608(02)00589-7.