PMID- 12505257
OWN - NLM
STAT- MEDLINE
DCOM- 20030115
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 138
IP  - 2
DP  - 2002 Oct 15
TI  - SKY detection of chromosome rearrangements in two cases of tMDS with a complex 
      karyotype.
PG  - 128-32
AB  - In this study, we used spectral karyotyping (SKY) and fluorescence in situ 
      hybridization (FISH) as complementary techniques for the analysis of two 
      therapy-related secondary myelodysplastic syndrome (t-MDS) cases with complex 
      karyotypes, previously analyzed by G-banding. Different types of SKY's 
      cytogenetic contributions include confirmation of G-banding results, 
      identification of partially characterized rearrangements, identification of 
      marker chromosomes unidentified by G-banding, and detection of cryptic reciprocal 
      translocations. In particular, the ability of SKY to clarify a number of markers 
      led to the comprehension of clonal evolution. The common aberration found in 
      these two t-MDS cases was the fragility of chromosome 5 and monosomy of 
      chromosome 18. We clearly present that the use of SKY combined with conventional 
      G-banding analysis and FISH has assisted in the identification of important 
      chromosomal events that may play a key role in the development of t-MDS.
FAU - Cohen, Ninette
AU  - Cohen N
AD  - Institute of Hematology and Pediatric Hemato-Oncology, The Chaim Sheba Medical 
      Center, Tel-Hashomer, Israel. ninettac@yahoo.com
FAU - Trakhtenbrot, Luba
AU  - Trakhtenbrot L
FAU - Yukla, Mona
AU  - Yukla M
FAU - Manor, Yosef
AU  - Manor Y
FAU - Gaber, Elena
AU  - Gaber E
FAU - Yosef, Gabi
AU  - Yosef G
FAU - Amariglio, Ninette
AU  - Amariglio N
FAU - Rechavi, Gideon
AU  - Rechavi G
FAU - Amiel, Aliza
AU  - Amiel A
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - *Chromosome Aberrations/chemically induced/radiation effects
MH  - Chromosomes, Human/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
MH  - Neoplasms, Second Primary/*genetics
MH  - Spectral Karyotyping
EDAT- 2002/12/31 04:00
MHDA- 2003/01/16 04:00
CRDT- 2002/12/31 04:00
PHST- 2002/12/31 04:00 [pubmed]
PHST- 2003/01/16 04:00 [medline]
PHST- 2002/12/31 04:00 [entrez]
AID - S0165460802005320 [pii]
AID - 10.1016/s0165-4608(02)00532-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Oct 15;138(2):128-32. doi: 
      10.1016/s0165-4608(02)00532-0.