PMID- 12505774
OWN - NLM
STAT- MEDLINE
DCOM- 20030731
LR  - 20190922
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 784
IP  - 2
DP  - 2003 Feb 5
TI  - Method for monitoring alginate released in biological fluids by high-performance 
      anion-exchange chromatography with pulsed amperometric detection.
PG  - 265-74
AB  - The use of alginate-entrapped cells in cell therapy requires a method for 
      monitoring possible released compound within biological fluids following either 
      their implantation or inoculation in artificial organs. Oligomannuronic and 
      oligoguluronic acids were prepared by enzymatic depolymerization with alginate 
      lyase from Pseudomonas alginovora, characterized by high-performance 
      anion-exchange chromatography with pulsed amperometric detection and quantitated 
      in human, pig and rabbit blood, urine and tissue samples. The method was tested 
      for linearity and detection limit, accuracy, intra- and inter-day precision. The 
      limit of detection was 3 microgram/ml in both urine and plasma and 5 mg/g of 
      tissues. The relative standard deviations (RSDs) of intra-day precision were 
      6.0-16.6% and 4.8-8.7% in plasma and urine, respectively; the RSDs of inter-day 
      precision were 5.1-14.4% and 5.0-11.6% in plasma and urine, respectively. Thus, 
      this method appears suitable for the measurement of released alginate from 
      entrapped cells used in cell therapy.
FAU - Desille, Mireille
AU  - Desille M
AD  - INSERM U456-Detoxication et Reparation Tissulaire, Universite de Rennes I, 
      Rennes, France.
FAU - Allain, Nathalie
AU  - Allain N
FAU - Anger, Jean Pierre
AU  - Anger JP
FAU - Mahler, Stephan
AU  - Mahler S
FAU - Lognone, Vincent
AU  - Lognone V
FAU - Malledant, Yannick
AU  - Malledant Y
FAU - Clement, Bruno
AU  - Clement B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Alginates)
RN  - 0 (Hexuronic Acids)
RN  - 8A5D83Q4RW (Glucuronic Acid)
SB  - IM
MH  - Alginates/*analysis/pharmacokinetics
MH  - Animals
MH  - Biological Availability
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Chromatography, Ion Exchange/*methods
MH  - Electrochemistry/*methods
MH  - Glucuronic Acid
MH  - Hexuronic Acids
MH  - Rabbits
MH  - Sensitivity and Specificity
EDAT- 2002/12/31 04:00
MHDA- 2003/08/02 05:00
CRDT- 2002/12/31 04:00
PHST- 2002/12/31 04:00 [pubmed]
PHST- 2003/08/02 05:00 [medline]
PHST- 2002/12/31 04:00 [entrez]
AID - S1570023202008012 [pii]
AID - 10.1016/s1570-0232(02)00801-2 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Feb 5;784(2):265-74. doi: 
      10.1016/s1570-0232(02)00801-2.