PMID- 12507852
OWN - NLM
STAT- MEDLINE
DCOM- 20030220
LR  - 20190901
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 91
IP  - 1
DP  - 2003 Jan 10
TI  - Immunohistochemical staining of IFN-gamma positive cells in porcine reproductive 
      and respiratory syndrome virus-infected lungs.
PG  - 73-7
AB  - Paraffin-embedded lungs were obtained from a previous porcine reproductive and 
      respiratory syndrome virus (PRRSV)-challenged experiment involving three groups: 
      an uninfected control group, a low virulence (LV, Resp PRRSV/Repro)-infected 
      group, and a high virulence (HV, VR-2385)-infected group. Tissues were collected 
      at 3, 7, 10, 14 or 28 days post-inoculation (DPI) (n=5). Lungs were examined to 
      detect IFN-gamma positive cells by immunohistochemical staining using polyclonal 
      antibodies to IFN-gamma. The microscopic lung lesions induced by the HV group 
      were more severe than those in the LV group. A significant increase in number of 
      lymphocytes in the HV group was observed at 10 DPI (24.90+/-9.79%), 14 DPI 
      (22.00+/-11.47%) and 28 DPI (28.95+/-15.11%) (P<0.05). A relative decrease in 
      macrophage numbers was observed and correlated well with the increase in 
      lymphocyte numbers when the disease progressed. IFN-gamma positive cells were 
      demonstrated in both lymphocytes and macrophages, particularly pulmonary alveolar 
      macrophages. A significant increase in IFN-gamma positive cells was found at 7 
      DPI (15.90+/-13.65%), 10 DPI (46.95+/-13.79%), 14 DPI (10.90+/-5.13%) and 28 DPI 
      (13.40+/-4.89%) in the HV group (P<0.05). The results suggested that the increase 
      in IFN-gamma positive cells in the HV group correlated well with the severity of 
      the lung lesions, which may be because of the presence of PRRSV in the lung.
FAU - Thanawongnuwech, Roongroje
AU  - Thanawongnuwech R
AD  - Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn 
      University, Bangkok 10330, Thailand. roongroje.t@chula.ac.th
FAU - Rungsipipat, Anudep
AU  - Rungsipipat A
FAU - Disatian, Sirilak
AU  - Disatian S
FAU - Saiyasombat, Rungrat
AU  - Saiyasombat R
FAU - Napakanaporn, Sudarat
AU  - Napakanaporn S
FAU - Halbur, Patrick G
AU  - Halbur PG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Immunohistochemistry
MH  - Interferon-gamma/*analysis
MH  - Lung/*immunology/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Swine
MH  - Virus Replication
EDAT- 2003/01/01 04:00
MHDA- 2003/02/21 04:00
CRDT- 2003/01/01 04:00
PHST- 2003/01/01 04:00 [pubmed]
PHST- 2003/02/21 04:00 [medline]
PHST- 2003/01/01 04:00 [entrez]
AID - S0165242702002684 [pii]
AID - 10.1016/s0165-2427(02)00268-4 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2003 Jan 10;91(1):73-7. doi: 
      10.1016/s0165-2427(02)00268-4.