PMID- 12509242
OWN - NLM
STAT- MEDLINE
DCOM- 20030305
LR  - 20121115
IS  - 1568-7864 (Print)
IS  - 1568-7856 (Linking)
VI  - 1
IP  - 5
DP  - 2002 May 30
TI  - Translation of hSNM1 is mediated by an internal ribosome entry site that 
      upregulates expression during mitosis.
PG  - 379-90
AB  - SNM1 is involved in the repair of DNA interstrand cross-links (ICLs) in 
      Saccharomyces cerevisiae and possibly in human cells, although relatively little 
      is known about its biochemical function. The hSNM1 contains a long 5' 
      untranslated region (5'UTR) predicted to fold into a complex secondary structure, 
      and which contains numerous short open reading frames (ORFs). We show here using 
      bicistronic constructs that human SNM1 mRNA contains an internal ribosome entry 
      site (IRES) that generally suppresses translation, except during mitosis where 
      translation is upregulated. These results suggest that hSNM1 may have a mitotic 
      function possibly involved in response to DNA interstrand cross-linking agents.
FAU - Zhang, Xiaoshan
AU  - Zhang X
AD  - The Department of Molecular Genetics, M.D. Anderson Cancer Center, The University 
      of Texas, Houston, TX 77030, USA.
FAU - Richie, Christopher
AU  - Richie C
FAU - Legerski, Randy J
AU  - Legerski RJ
LA  - eng
GR  - CA09299/CA/NCI NIH HHS/United States
GR  - CA52461/CA/NCI NIH HHS/United States
GR  - CA75160/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - DNA Repair (Amst)
JT  - DNA repair
JID - 101139138
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.1.- (Endodeoxyribonucleases)
RN  - EC 3.1.- (PSO2 protein, S cerevisiae)
SB  - IM
MH  - 5' Untranslated Regions/*chemistry/physiology
MH  - Cell Division
MH  - Cells, Cultured
MH  - DNA Damage
MH  - DNA Primers/chemistry
MH  - DNA Repair
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Endodeoxyribonucleases
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins/metabolism
MH  - Mitosis/*physiology
MH  - Nuclear Proteins/*genetics/metabolism
MH  - Nucleic Acid Conformation
MH  - Open Reading Frames
MH  - Polymerase Chain Reaction
MH  - *Protein Biosynthesis
MH  - RNA, Messenger/genetics/metabolism
MH  - Ribosomes/*metabolism
MH  - Saccharomyces cerevisiae Proteins/*genetics/metabolism
MH  - Transfection
MH  - Up-Regulation
EDAT- 2003/01/02 04:00
MHDA- 2003/03/06 04:00
CRDT- 2003/01/02 04:00
PHST- 2003/01/02 04:00 [pubmed]
PHST- 2003/03/06 04:00 [medline]
PHST- 2003/01/02 04:00 [entrez]
AID - S1568786402000150 [pii]
PST - ppublish
SO  - DNA Repair (Amst). 2002 May 30;1(5):379-90.