PMID- 12511415
OWN - NLM
STAT- MEDLINE
DCOM- 20030620
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 101
IP  - 9
DP  - 2003 May 1
TI  - Reconstitution of NK cell receptor repertoire following HLA-matched hematopoietic 
      cell transplantation.
PG  - 3730-40
AB  - Interactions between killer immunoglobulin-like receptors (KIRs) and human 
      leukocyte antigen (HLA) class I ligands influence development of natural killer 
      (NK) cell repertoire and response to infection, cancer, and allogeneic tissue. As 
      KIRs and HLA class I molecules are highly polymorphic, clinical allogeneic 
      hematopoietic cell transplantation is predicted to frequently involve KIR 
      mismatch, and thus to provide a unique system for study of human NK cell receptor 
      repertoire development. Eighteen leukemia patients undergoing HLA-matched 
      transplantation and their donors were analyzed for KIR genotype. Ten of 13 
      HLA-identical donor-patient pairs were KIR mismatched and 3 were matched; all 
      HLA-matched unrelated pairs were KIR mismatched. Reconstitution of recipient NK 
      cell repertoire following transplantation was examined using flow cytometry and 
      monoclonal antibodies specific for KIR and CD94:NKG2A. These data form 3 groups. 
      Six to 9 months after transplantation, 8 patients (group 1) reconstituted an NK 
      cell repertoire resembling that of their donor, and for KIR-mismatched 
      transplants, distinct from the recipient before transplantation. In the first 
      year after transplantation, 5 patients (group 2) exhibited a generally depressed 
      frequency of KIR-expressing NK cells and concomitant high frequency of CD94:NKG2A 
      expression. By 3 years after transplantation, the frequency of KIR-expressing NK 
      cells had increased to donor values, in the 3 patients from group 2 analyzed for 
      this period. The remaining 5 patients experienced severe clinical complications 
      following transplantation and displayed unique features in their NK cell receptor 
      reconstitution. These results demonstrate that a majority of HLA-matched 
      hematopoietic cell transplantations involve KIR mismatch and reveal differences 
      in NK cell repertoire having potential impact for immune responsiveness and 
      transplantation outcome.
FAU - Shilling, Heather G
AU  - Shilling HG
AD  - Department of Structural Biology, Stanford University School of Medicine, 
      Stanford, CA 94305, USA.
FAU - McQueen, Karina L
AU  - McQueen KL
FAU - Cheng, Nathalie W
AU  - Cheng NW
FAU - Shizuru, Judith A
AU  - Shizuru JA
FAU - Negrin, Robert S
AU  - Negrin RS
FAU - Parham, Peter
AU  - Parham P
LA  - eng
GR  - AI07290/AI/NIAID NIH HHS/United States
GR  - CA49605/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030102
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (HLA Antigens)
RN  - 0 (KLRC1 protein, human)
RN  - 0 (KLRD1 protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily C)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily D)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, KIR)
RN  - 0 (Receptors, Natural Killer Cell)
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Antigens, CD/*genetics/immunology
MH  - Cell Lineage
MH  - Cytotoxicity, Immunologic
MH  - Female
MH  - Follow-Up Studies
MH  - Genotype
MH  - Graft Survival
MH  - Graft vs Host Disease/etiology/immunology
MH  - HLA Antigens/genetics
MH  - Histocompatibility
MH  - Humans
MH  - Killer Cells, Natural/*cytology/immunology
MH  - Lectins, C-Type/*genetics/immunology
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/therapy
MH  - Leukemia, Myeloid/therapy
MH  - Male
MH  - Middle Aged
MH  - NK Cell Lectin-Like Receptor Subfamily C
MH  - NK Cell Lectin-Like Receptor Subfamily D
MH  - *Peripheral Blood Stem Cell Transplantation/adverse effects
MH  - Receptors, Immunologic/*genetics/immunology
MH  - Receptors, KIR
MH  - Receptors, Natural Killer Cell
MH  - Remission Induction
MH  - Transplantation Chimera
MH  - Transplantation, Homologous/adverse effects/immunology
MH  - Treatment Outcome
EDAT- 2003/01/04 04:00
MHDA- 2003/06/21 05:00
CRDT- 2003/01/04 04:00
PHST- 2003/01/04 04:00 [pubmed]
PHST- 2003/06/21 05:00 [medline]
PHST- 2003/01/04 04:00 [entrez]
AID - S0006-4971(20)50762-3 [pii]
AID - 10.1182/blood-2002-08-2568 [doi]
PST - ppublish
SO  - Blood. 2003 May 1;101(9):3730-40. doi: 10.1182/blood-2002-08-2568. Epub 2003 Jan 
      2.