PMID- 12513760
OWN - NLM
STAT- MEDLINE
DCOM- 20030723
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 10
IP  - 4
DP  - 2002 Aug
TI  - Regulative function of extracellular regulated protein kinases and telomerase in 
      apoptosis of hepatocarcinomatous and leukemic cell lines.
PG  - 294-8
AB  - In order to investigate the change of telomerase activity and phosphorylated 
      (activated) extracellular regulated protein kinases (ERK) 1 and 2 in 
      hepatocarcinomatous cell line SMMC7721 and leukemic cell line K562 proliferation 
      inhibition and apoptosis, three kinds of chemotherapeutic drugs harringtonine 
      (HRT), vincristine (VCR) and etoposide (VP-16) were selected as inducers; and MTT 
      assay, flow cytometry analysis, telomeric repeat amplification protocol (TRAP) 
      assay and bioluminescence analysis were used. The results showed that after 
      treatment of HRT, VCR and VP-16 for 24 hours, the cell proliferation was 
      inhibited, apoptosis was induced, and telomerase activity and the protein 
      expression of phosphorylated ERK1/2 were down-regulated. In HRT treated groups, 
      the descendent grade was the most obvious. It was concluded that the common 
      molecular mechanism of these chemotherapeutic drugs killing SMMC7721 and K562 
      cell lines might be through inhibiting ERK signal transduction pathways, cutting 
      down ERK activity, reducing the transcription of target genes of ERKs, then 
      indirectly down-regulate telomerase activity, and cell apoptosis is the final 
      result of durative loss of telomere.
FAU - Li, Deng-Ju
AU  - Li DJ
AD  - Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China. lidengju@163.net
FAU - Zhang, Yao-Zhen
AU  - Zhang YZ
FAU - Meng, Fan-Kai
AU  - Meng FK
FAU - Zhang, Dong-Hua
AU  - Zhang DH
FAU - Liu, Wen-Li
AU  - Liu WL
LA  - eng
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Antineoplastic Agents)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - *Apoptosis
MH  - Carcinoma, Hepatocellular/*enzymology/pathology
MH  - Humans
MH  - K562 Cells
MH  - Leukemia/*enzymology/pathology
MH  - Liver Neoplasms/*enzymology/pathology
MH  - Mitogen-Activated Protein Kinase 1/*metabolism
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Phosphorylation
MH  - Telomerase/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 2003/01/07 04:00
MHDA- 2003/07/24 05:00
CRDT- 2003/01/07 04:00
PHST- 2003/01/07 04:00 [pubmed]
PHST- 2003/07/24 05:00 [medline]
PHST- 2003/01/07 04:00 [entrez]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2002 Aug;10(4):294-8.