PMID- 12513764
OWN - NLM
STAT- MEDLINE
DCOM- 20030723
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 10
IP  - 4
DP  - 2002 Aug
TI  - [Study on angiogenesis of multiple myeloma in vitro].
PG  - 310-4
AB  - Angiogenesis is a necessary step in tumor progression, and it correlates an 
      unfavorable prognosis. In multiple myeloma, bone marrow microvessel density and 
      angiogenesis grading correlated with plasma cell labeling index and are poor 
      survival predictors, but the study of myeloma's angiogenesis is very rare. This 
      article was to study the effect of multiple myeloma cell line conditioned media 
      on the proliferation, migration and angiogenesis of human bone marrow endothelial 
      cells (HBMEC). The multiple myeloma cell line conditioned media were obtained by 
      using RPMI 1640 media containing 2% fetal bovine serum (FBS) to cultivate myeloma 
      cell lines for 18 hours. Proliferation and migration of HBMEC were detected by 
      using those media to cultivate HBMEC. Capillary tube formation was performed by 
      using microcarriers cytodex-3 covered with HBMEC in three-dimensional fibrin 
      matrices. The results showed that myeloma conditioned media induced HBMEC's 
      proliferation and migration (P < 0.001), and those media induced capillary tube 
      formation (length and width) of HBMEC (P < 0.001). It was concluded that myeloma 
      cell lines induce HBMEC's proliferation, migration, and capillary tube formation 
      by secreting several cytokines.
FAU - Chen, Wen-Ming
AU  - Chen WM
AD  - Department of Hematology, Beijing Chaoyang Hospital, Capital University of 
      Medical Sciences, Beijing 100020, China. wenming-chen@yahoo.com
FAU - Wu, Yin
AU  - Wu Y
FAU - Zhu, Jia-Zhi
AU  - Zhu JZ
FAU - Soria, Jeannette
AU  - Soria J
FAU - Mirshahi, Massoud
AU  - Mirshahi M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lymphokines)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
SB  - IM
MH  - Bone Marrow Cells/cytology
MH  - Cell Division
MH  - Cell Movement
MH  - Endothelial Growth Factors/analysis/physiology
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/analysis/physiology
MH  - Lymphokines/analysis/physiology
MH  - Multiple Myeloma/*blood supply/chemistry/pathology
MH  - Neovascularization, Pathologic/*etiology
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 2003/01/07 04:00
MHDA- 2003/07/24 05:00
CRDT- 2003/01/07 04:00
PHST- 2003/01/07 04:00 [pubmed]
PHST- 2003/07/24 05:00 [medline]
PHST- 2003/01/07 04:00 [entrez]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2002 Aug;10(4):310-4.